Articles
DETECTION AND IDENTIFICATION OF TOSPOVIRUSES IN GREENHOUSES
Article number
431_8
Pages
90 – 98
Language
Abstract
Of the greenhouse-grown flower samples submitted to our laboratory from 1989 to 1995, 5–15% annually have been identified as tospovirus infested.
Since 1990, enzyme-linked immunosorbent assay (ELISA) has been used to differentiate tospoviruses.
Only 18% of tospovirus-positive samples have been identified as tomato spotted wilt virus (TSWV); impatiens necrotic ringspot virus (INSV) predominates.
Symptoms observed on 31 flower crops have included ringspots, stem cankers, pale white or yellow spots, necrotic spots, chlorotic mottle, leaf-base necrosis, necrotic or chlorotic line patterns, and stunting.
These often mimic symptoms of other contagious diseases.
A tospovirus has been detected in Diascia, Platycodon, and Scaevola, which have not been reported as hosts of tospoviruses.
Since 1990, enzyme-linked immunosorbent assay (ELISA) has been used to differentiate tospoviruses.
Only 18% of tospovirus-positive samples have been identified as tomato spotted wilt virus (TSWV); impatiens necrotic ringspot virus (INSV) predominates.
Symptoms observed on 31 flower crops have included ringspots, stem cankers, pale white or yellow spots, necrotic spots, chlorotic mottle, leaf-base necrosis, necrotic or chlorotic line patterns, and stunting.
These often mimic symptoms of other contagious diseases.
A tospovirus has been detected in Diascia, Platycodon, and Scaevola, which have not been reported as hosts of tospoviruses.
Authors
M.L. Daughtrey
Keywords
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