Articles
BREEDING TRANSGENIC TOMATOES FOR RESISTANCE TO TOMATO SPOTTED WILT VIRUS AND CUCUMBER MOSAIC VIRUS
Article number
431_40
Pages
442 – 448
Language
Abstract
We have transferred the nucleocapsid protein gene of the lettuce isolate of tomato spotted wilt virus (TSWV-BL) into a tobacco mosaic virus (TMV) resistant tomato (Lycopersicon esculentum) line, (Geneva 80), via Agrobacterium tumefaciens.
Kanamycin-resistant transgenic R0 lines were selfed and their progenies were resistant to the homologous TSWV-BL isolate.
Transgenic R1 plants were also tested for their ability to resist infections by other tospoviruses.
In addition, R2 lines were obtained from TSWV-BL-resistant R1 plants.
One of these lines was crossed with a cucumber mosaic virus (CMV) resistant transgenic Geneva 80 line that is homozygous for the coat protein gene of CMV. Their progenies were resistant to TSWV and CMV.
Kanamycin-resistant transgenic R0 lines were selfed and their progenies were resistant to the homologous TSWV-BL isolate.
Transgenic R1 plants were also tested for their ability to resist infections by other tospoviruses.
In addition, R2 lines were obtained from TSWV-BL-resistant R1 plants.
One of these lines was crossed with a cucumber mosaic virus (CMV) resistant transgenic Geneva 80 line that is homozygous for the coat protein gene of CMV. Their progenies were resistant to TSWV and CMV.
Authors
C. Gonsalves, B. Xue, S.Z. Pang, R. Provvidenti, J.L. Slightom, D. Gonsalves
Keywords
Online Articles (51)
