Articles
MORPHOGENIC RESPONSES AND IN VITRO REGENERATION OF CANELO (DRIMYS WINTERI J.R. ET FORSTER), A FOREST SPECIES USED IN CHILEAN TRADITIONAL MEDICINE
Article number
502_46
Pages
289 – 294
Language
English
Abstract
Drimys winteri (canelo) is an important native forest tree common to southern latitudes of Chile and Argentina.
Micropropagation and selection work of this species is important for reforestation; however, best characters from different populations have to be determined, fixed, and asexually multiplied for use.
The aim of the present work is to evaluate the regeneration potential being expressed by different in vitro cultured explants of this species.
Using shoot-tips, root formation was obtained leading to plantlet regeneration.
Rooting was obtained in a two-step culture, the first consisted in a WPM-liquid medium, including 1.61 μM
-naphthaleneacetic acid (NAA), 0.44 μM 6-benzyladenine (BA) and 0.03 μM of gibberellic acid (GA3) for 60 days at low light intensity (10 μmol m-2 s-1) followed by a subculture in half-strength liquid MS-medium in presence of 26.85 μM NAA and 0.5 mg/l Ca-panthotenate.
Roots were not formed in permanent darkness.
Shoot organogenesis and multiple shoot formation were obtained from internodal explants and internodal-derived callus respectively.
Single shoots were commonly observed in internodes after 1–2 months and multiple shoots formed mainly on callus after 4 months, in presence of 0.54, 5.37 and 53.71 μM NAA or 0.46 and 4.65 μM 6-furfurylaminopurine (K), in 90% of explants.
Under continuous darkness, callus formed more than 40 shoots/explant.
Direct shoot-formation also occurred in leaf sections up to 70%, in presence of 0.45 or 11.35 μM thidiazuron (TDZ) in combination with 0.54 or 5.37 μM NAA.
Micropropagation and selection work of this species is important for reforestation; however, best characters from different populations have to be determined, fixed, and asexually multiplied for use.
The aim of the present work is to evaluate the regeneration potential being expressed by different in vitro cultured explants of this species.
Using shoot-tips, root formation was obtained leading to plantlet regeneration.
Rooting was obtained in a two-step culture, the first consisted in a WPM-liquid medium, including 1.61 μM
-naphthaleneacetic acid (NAA), 0.44 μM 6-benzyladenine (BA) and 0.03 μM of gibberellic acid (GA3) for 60 days at low light intensity (10 μmol m-2 s-1) followed by a subculture in half-strength liquid MS-medium in presence of 26.85 μM NAA and 0.5 mg/l Ca-panthotenate.Roots were not formed in permanent darkness.
Shoot organogenesis and multiple shoot formation were obtained from internodal explants and internodal-derived callus respectively.
Single shoots were commonly observed in internodes after 1–2 months and multiple shoots formed mainly on callus after 4 months, in presence of 0.54, 5.37 and 53.71 μM NAA or 0.46 and 4.65 μM 6-furfurylaminopurine (K), in 90% of explants.
Under continuous darkness, callus formed more than 40 shoots/explant.
Direct shoot-formation also occurred in leaf sections up to 70%, in presence of 0.45 or 11.35 μM thidiazuron (TDZ) in combination with 0.54 or 5.37 μM NAA.
Authors
M. Jordan
Keywords
Drimys winteri, medicinal plant, multiple shoots, organogenesis, tissue culture
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