Home > II WOCMAP Congress Medicinal and Aromatic Plants, Part 3: Agricultural Production, Post Harvest Techniques, Biotechnology > COUMARIN PRODUCTION IN MIKANIA GLOMERATA CALLUS

Articles

COUMARIN PRODUCTION IN MIKANIA GLOMERATA CALLUS

Article number

502_59

Pages

357 – 362

Language

English

Abstract

Cell cultures of Mikania glomerata Sprengel were established with leaf segments cultured on White medium supplemented with 1 mg/L BA and 3 mg/L NAA. Different types and concentrations of growth regulators were tested for callus maintenance.
Determination of coumarin content was performed in HPLC using authentic coumarin standard.
Growth regulator concentration affected biomass and coumarin accumulation.
Cultures developed in semisolid medium containing both BA and NAA exhibited enhanced biomass production as well as coumarin accumulation.
In the most favorable conditions tested, cells accumulated 25 μg/g of dry weight what is much inferior to the yield already reported in intact plants (5 mg/g of dry weight). However, results obtained so far suggest several alternatives for culture manipulation in order to optimize the productivity of coumarin by M. glomerata cultured cells.

Publication

II WOCMAP Congress Medicinal and Aromatic Plants, Part 3: Agricultural Production, Post Harvest Techniques, Biotechnology

Authors

A.M.S. Pereira, S.C. França, F.L.A. Câmara

Keywords

guaco, Mikania glomerata, tissue culture, medicinal plant

Full text

https://doi.org/10.17660/ActaHortic.1999.502.59

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