Articles
MICROPROPAGATION OF ARISTOLOCHIA FIMBRIATA CHAM.
Article number
502_56
Pages
339 – 346
Language
English
Abstract
A method for micropropagation of Aristolochia fimbriata Cham. is described.
The basal medium contained the Murashige-Skoog’s salts (MS), Gamborg’s vitamins and sucrose.
One-node stem segments were initiated on the basal medium supplemented with 0.1 mg l-1 gibberellic acid (GA3), 2.5 mg l-1 6-benzyl-amino purine (BAP) and indole-3- butyric acid (IBA) at different concentrations, achieving 5-fold shoot multiplication at 14 days with 0.25 mg l-1 IBA. The formed shoots were sectioned and transferred to the basal medium with BAP (1.0 mg l-1) where 8-fold shoot multiplication was obtained.
For root formation, shoots were cultured on the basal medium with half the MS macronutrients, supplemented with IBA or a-naphthalene-acetic acid (NAA). The IBA supplementation produced roots in all the tested concentrations, while NAA only led to callus formation.
The basal medium contained the Murashige-Skoog’s salts (MS), Gamborg’s vitamins and sucrose.
One-node stem segments were initiated on the basal medium supplemented with 0.1 mg l-1 gibberellic acid (GA3), 2.5 mg l-1 6-benzyl-amino purine (BAP) and indole-3- butyric acid (IBA) at different concentrations, achieving 5-fold shoot multiplication at 14 days with 0.25 mg l-1 IBA. The formed shoots were sectioned and transferred to the basal medium with BAP (1.0 mg l-1) where 8-fold shoot multiplication was obtained.
For root formation, shoots were cultured on the basal medium with half the MS macronutrients, supplemented with IBA or a-naphthalene-acetic acid (NAA). The IBA supplementation produced roots in all the tested concentrations, while NAA only led to callus formation.
Authors
C. Bravo, G. Yormann, B. Llorente
Keywords
Aristolochia fimbriata, Aristolochiaceae, aristolochic acids, patito
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