EFFECTS OF AIR EXCHANGE, SUCROSE, AND PPF ON GROWTH OF REHMANNIA GLUTINOSA UNDER ENRICHED CO₂ CONCENTRATION IN VITRO

The explants excised from Rehmannia glutinosa plantlets were cultured on Murashige-Skoog (MS) medium with or without sucrose under various environmental conditions such as air exchanges, photosynthetic photon flux (PPF) and CO₂ enrichment.
Healthy plantlets, which had normal stomatal activity and high survival rate were obtained by increasing the air exchange rates per hour in culture vessel.
More than two times of leaf area and dry weight were observed compared with conventional tissue culture method under increased air exchange rate, high PPF and CO₂ enriched conditions.
Although the presence of sucrose led to the maximal growth, the plantlets cultured on sucrose-free medium showed fair growth characteristics.
It was clearly shown that plantlets grown under sucrosefree, CO₂ enrichment and high PPF conditions had autotrophic growth characteristics.

II WOCMAP Congress Medicinal and Aromatic Plants, Part 3: Agricultural Production, Post Harvest Techniques, Biotechnology

J.H. Seon, C.H. Cui, K.Y. Paek, C.S. Yang, W.Y. Gao, C.H. Park, S.N. Sung

Rehmannia glutinosa, autotrophic, mixotrophic, heterotrophic, stomatal diffusive resistance

https://doi.org/10.17660/ActaHortic.1999.502.51