Articles
MICROPROPAGATION OF ACHYRANTHES JAPONICA THROUGH AXILLARY BUDS CULTURE
Article number
502_54
Pages
329 – 332
Language
English
Abstract
Multiple shoot formation was obtained from excised axillary buds of Achyranthes japonica NAKAI cultured on Murashige & Skoog (MS) media containing various growth regulators such as auxin and cytokinin.
The highest average number of shoots was obtained in 1 mg/l
-naphthalene acetic acid (NAA) and 2 mg/l 6-benzyladenine (BA) after 6 weeks (25.8 adventitious shoots per node). Although the regeneration rate was less than the former condition, optimal combination for the production of more shoots with a suitable size was 0.5 mg/l NAA and 1.0 mg/l BA (19.7 adventitious shoots per node). Roots were induced from regenerated shoots after 3 weeks culture, transferred to ½ MS medium supplemented with 0.1 mg/l indole butyric acid (IBA). Micropropagated plants were successfully transferred to soil.
The highest average number of shoots was obtained in 1 mg/l
-naphthalene acetic acid (NAA) and 2 mg/l 6-benzyladenine (BA) after 6 weeks (25.8 adventitious shoots per node). Although the regeneration rate was less than the former condition, optimal combination for the production of more shoots with a suitable size was 0.5 mg/l NAA and 1.0 mg/l BA (19.7 adventitious shoots per node). Roots were induced from regenerated shoots after 3 weeks culture, transferred to ½ MS medium supplemented with 0.1 mg/l indole butyric acid (IBA). Micropropagated plants were successfully transferred to soil.
Authors
K.S. Kim, B. Hwang, M.W. Kim, N.S. Seung
Keywords
Adventitious shoot, Medicinal plant, Achyranthes japonica, Micropropagation
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