Articles

ROOT ROT OF YOUNG OLIVE TREES CAUSED BY PHYTOPHTHORA PALMIVORA IN SOUTHERN ITALY

Article number
586_151
Pages
709 – 712
Language
English
Abstract
In summer 1999, young trees of olive cv.
Carolea showing decline symptoms were observed in young commercial plantations in Calabria (Southern Italy). This syndrome was associated with a severe root rot. Phytophthora palmivora was consistently isolated from roots of symptomatic trees.
The pathogen was identified primarily on the basis of morphological and cultural characters.
The isolates produced elliptical to ovoid, prominently papillate, caducous (mean pedicel length 5 mm) sporangia, with a mean length-breadth ratio of 1.8. Some isolates produced, besides these sporangia, non-papillate and subglobose sporangia (length-breadth ratio ranging from 1.2 to 1.5) morphologically similar to the sporocysts of P. palmivora var. heterocystica. The species identification was confirmed by polyacrylamide slab gel electrophoresis of mycelial proteins.
The electrophoretic banding patterns of total soluble proteins and eight isozymes of both types of isolates were identical to those shown by two reference isolates of P. palmivora. Conversely, they were clearly distinct from the electrophoretic banding patterns of isolates of other papillate species of Phytophthora used as reference.
Pairings with isolates of known mating type of P. capsici, P. nicotianae and P. palmivora showed that all the isolates of P. palmivora from olive were A1 mating type.
Koch’s postulates were completed using 6-month-old rooted cuttings of cv.
Carolea and 1-yr-old trees of cv.
Biancolilla grafted onto wild olive.

Publication
Authors
G.E. Agosteo, G. Magnano di San Lio, S.O. Cacciola
Keywords
Phytophthora palmivora, morphology, mycelial proteins, polyacrylamide gel electrophoresis, isozymes
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