Articles

IN PLANTA-PCR DETECTION OF OLIVE-DEFOLIATING AND NON DEFOLIATING PATHOTYPES OF VERTICILLIUM DAHLIAE

Article number
586_163
Pages
757 – 760
Language
English
Abstract
Verticillium wilt, caused by Verticillium dahliae Kleb., affects olive orchards throughout the range of cultivation.
Severity of disease attacks is highly influenced by the nature of V. dahliae isolates, which can be classified into defoliating (D) and nondefoliating (ND), according to their ability to completely defoliate cotton and olive or to cause only mild wilt and no defoliation, respectively.
In Spain, an increasing incidence and distribution of Verticillium wilt of olive occurred in the last few years, especially in young and newly established olive orchards.
One of the most effective measures for the integrated management of this disease is the early in-planta detection of the pathogen so as to facilitate implementation of certification schemes for pathogen-free planting material.
A PCR-based procedure has been developed for the consistent detection of D and ND pathotypes of V. dahliae in infected olive plants.
The procedure involves a methodology for extracting template DNA from infected olive tissue, the development of pathotype-specific primers, and optimization of PCR reactions.
By the combined use of specific primers and different nested-PCR protocols, the detection of each of the two pathotypes of V. dahliae was obtained both in root and stem tissues of young nursery olive plants.
Detection of the pathogen was possible very early following artificial inoculation of olive plants with a conidial suspension of the pathogen.
Detection was also possible in symptomless olive plants as well as in plants was apparently recovered from infection by the ND pathotype.

Publication
Authors
J. Mercado-Blanco, D. Rodríguez-Jurado, E. Pérez-Artés, R.M. Jiménez-Díaz
Keywords
Olea europaea, Verticillium wilt, nested-PCR, genetic markers, plant health- certification programs
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