Home > IX International Symposium on Flower Bulbs > PLANT REGENERATION AND CHROMOSOME DOUBLING OF WILD GLADIOLUS SPECIES

Articles

PLANT REGENERATION AND CHROMOSOME DOUBLING OF WILD GLADIOLUS SPECIES

Article number

673_20

Pages

175 – 181

Language

English

Abstract

A plant regeneration system from callus and chromosome doubling by colchicine treatment was investigated in wild Gladiolus species.
Corm slices of 3 species, G. priorii, G. tristis and G. virescens, were used and callus was induced in liquid MS medium supplemented with BA or 2,4-D. The resulting callus was classified as two types – a friable type induced by BA and a compact type induced by 2,4-D. The former callus easily regenerated shoots with further cultivation.
Chromosome doubling was induced by treating callus initiating shoots with colchicine.
The highest efficiency of chromosome doubling was observed in the treatment with colchicine at 0.05% for 72 hours.
The regenerants of the wild species having doubled chromosomes will be powerful materials for gladiolus breeding.

Publication

IX International Symposium on Flower Bulbs

Authors

K. Suzuki, Y. Takatsu, T. Gonai, M. Kasumi

Keywords

gladiolus, wild species, callus, plant regeneration, chromosome doubling, colchicine

Full text

https://doi.org/10.17660/ActaHortic.2005.673.20

Online Articles (110)

2. GLOBALISATION – FLOWER – FLOWER BULBS – BULB FLOWERS

J.C.M. Buschman

3. PRODUCTION OF FLOWER BULBS AND BULBOUS CUT FLOWERS IN JAPAN – PAST, PRESENT, AND FUTURE

K. Ohkawa

4. BULBOUS MONOCOTS NATIVE TO JAPAN AND ADJACENT AREAS – THEIR HABITATS, LIFE HISTORIES AND PHYLOGENY

K. Hayashi | S. Kawano

5. PRODUCTION OF FLOWER BULBS IN REGIONS WITH WARM CLIMATES

R. Kamenetsky

6. PLATFORM APPROACH FOR SUSTAINABLE FLOWERBULB PRODUCTION

S. de Kool | A. Krikke

7. INTEGRATED MANAGEMENT OF SOIL-BORNE DISEASE IN FLOWER BULB PRODUCTION

8. THE CONTRIBUTION OF QUALITY INSPECTIONS TO THE IMPROVEMENT OF THE QUALITY OF THE DUTCH FLOWERBULBS AND ACCESS TO EXPORT MARKETS

P.J.M. Knippels

9. CA DURING CHILLING STORAGE SUPPRESSES THE INDUCTION OF DAUGHTER BULB ENLARGEMENT IN TULIPS

K. Inamoto | S. Sakoda | T. Umemoto | M. Doi | H. Imanishi

10. STUDIES ON THE BULB DEVELOPMENT AND ITS PHYSIOLOGICAL MECHANISMS IN LILIUM ORIENTAL HYBRIDS

Y.P. Xia | H.J. Zheng | C.H. Huang

11. MEIOTIC POLYPLOIDIZATION IN FIVE DIFFERENT INTERSPECIFIC LILIUM HYBRIDS

J.M. van Tuyl | R. Barba-Gonzalez | A.A. van Silfhout | K.-B. Lim | M.S. Ramanna

12. IMPROVEMENT OF BREEDING EFFICIENCY FOR INTERSPECIFIC HYBRIDIZATION OF LILIES IN KOREA

H.K. Rhee | J.H. Lim | Y.J. Kim | J.M. van Tuyl

13. CHARACTER VARIATION AND A CLADISTIC ANALYSIS OF THE GENUS LACHENALIA JACQ. F. EX MURRAY (HYACINTHACEAE)

G.D. Duncan | T.J. Edwards | A. Mitchell

14. PROPAGATION STUDIES ON CHILEAN GEOPHYTES

F. Schiappacasse | P. Peñailillo | P. Yáñez | M. Bridgen

15. NEW ASPECTS OF TULIP BREEDING: EMBRYO CULTURE AND POLYPLOID

K. Okazaki

16. BREEDING OF AUTUMN-FLOWERING INTERSPECIFIC HYBRIDS OF ALLIUM

Y. Nomura | M. Saito | H. Komori

17. THE BREEDING IN POLYKARYOMORPHIC PROGENIES OF SPIDER LILY (LYCORIS SPP.) MEDIATED SYNTHETIC DIKARYOTYPE HYBRIDS 2n+18 (4M+3T+11A)

M.C. Wu | M.S. Yuan | C.T. Shii

18. PHYLOGENETIC RELATIONSHIP OF TURKISH TERRESTRIAL ORCHIDS

E. Ari | O. Karaguzel | K. Onal | I. Polat | M. Gocmen

19. USE OF 2n GAMETES FOR INDUCING INTERGENOMIC RECOMBINATION IN LILY HYBRIDS

R. Barba-Gonzalez | K.-B. Lim | M.S. Ramanna | J.M. van Tuyl

20. CHARACTERISTICS OF THE TETRAPLOID PLANT DERIVED AS A SOMACLONAL VARIATION IN LILIUM LONGIFLORUM

R. Sangthong | M. Mii | P. Soonthornchainaksaeng | K. Supaibulwatana

21. PLANT REGENERATION AND CHROMOSOME DOUBLING OF WILD GLADIOLUS SPECIES

K. Suzuki | Y. Takatsu | T. Gonai | M. Kasumi

22. PARTICLE BOMBARDMENT-MEDIATED TRANSFORMATION OF ORNITHOGALUM DUBIUM FOR ORNITHOGALUM MOSAIC VIRUS RESISTANCE

23. HYMENOCALLIS SALISB. GERMINATION VARIANTS AND SEEDLING YIELD

M.W. Borys | H. Leszczynska-Borys | J.L. Galván

24. AUXIN TRANSPORT AND STEM GROWTH IN TULIPS: THE ROLE OF EPIDERMIS, CORTEX AND PITH

M. Saniewski | J. Ueda | K. Miyamoto | H. Okubo

25. EFFECTS OF TIBA ON GROWTH AND FLOWERING OF NON PRE-COOLED TULIP BULBS

X.M. Geng | K. Li-Nagasuga | H. Okubo | M. Saniewski

26. GUM FORMATION IN TULIPS AFFECTED BY METHYL JASMONATE REQUIRES CHANGES IN SUGAR METABOLISM

E. Skrzypek | K. Miyamoto | M. Saniewski | N. Higuchi | J. Ueda

27. CHARACTERISTICS OF ALPHA-AMYLASE IN BULB SCALES OF TULIP (TULIPA GESNERIANA L.)

28. VARIATION IN FUSARIUM-INDUCED ETHYLENE PRODUCTION AMONG TULIP CULTIVARS

29. POST-HEAT STRESS RESPIRATION PATTERN OF TULIP BULBS IN STORAGE

S.S. Liou | C.B. Watkins | W.B. Miller

30. THE USE OF 1-MCP AS AN INHIBITOR OF ETHYLENE ACTION IN TULIP BULBS UNDER LABORATORY AND PRACTICAL CONDITIONS

H. Gude | M. Dijkema

31. INFLUENCE OF DRYING CONDITIONS AND STORAGE TEMPERATURE ON DESICCATION AND LOSS OF TUBERS DURING STORAGE AND SUBSEQUENT GROWTH OF ZANTEDESCHIA TUBERS

P.J. van Leeuwen | J.P.T. Trompert

32. THE EFFECT OF GIBBERELLIN AND CYTOKININ ON FLORAL DEVELOPMENT IN ZANTEDESCHIA SPP. IN VIVO AND IN VITRO

V. Naor | J. Kigel | M. Ziv

33. EFFECTS OF PLANTING DATE AND ENVIRONMENT ON THE CUT FLOWER PRODUCTION OF SANTONIA ‘GOLDEN LIGHTS’

G.E. Clark | G.K. Burge | E.R. Morgan | C.M. Triggs

34. FLOWERING OF ZANTEDESCHIA REHMANNII ENGL. AS AFFECTED BY COMBINATION OF TUBER STORAGE TEMPERATURE AND DURATION

T. Goto | K. Kawajiri | Y. Kageyama | K. Konishi

35. FLOWERING ADVANCEMENT IN HERBACEOUS PEONY

A.H. Halevy | A. Barzilay | R. Kamenetsky

36. EFFECT OF ALTERNATING AND CONSTANT TEMPERATURE ON SEED GERMINATION OF CHELCHERAGH LILIY (LILIUM LEDEBOURII) IN IRAN

M.N. Padasht Dehkaei | A. Khalighi | R. Naderi | A. Moosavi

37. LILIUM LONGIFLORUM THUNB., A CLASSIC MODEL TO STUDY TEMPERATURE AND PHOTOPERIOD INTERACTIONS ON DORMANCY, FLOWER INDUCTION, LEAF UNFOLDING AND FLOWER DEVELOPMENT

H.F. Wilkins

38. GROWTH AND QUALITY OF ORIENTAL LILIES AT DIFFERENT FERTILIZATION LEVELS

J. Treder

39. CONTROLLING PLANT HEIGHT AND UPPER LEAF NECROSIS IN ORIENTAL LILIES BY ‘NEGATIVE DIF’

H. Kok | H. van Aanholt | H. Gude

40. EFFECT OF CERTAIN PRE-TREATMENT SUBSTANCES ON VASE LIFE AND PHYSIOLOGICAL CHARACTER IN LILIUM SPP.

J.-H. Kim | A.-K. Lee | J.-K. Suh

41. DEVELOPMENT OF FLORAL ORGAN AND PHYSIOCHEMICAL CHANGES OF CUT IRIS HOLLANDICA ‘BLUE MAGIC’ ACCORDING TO PLANT GROWTH REGULATORS AND STORAGE TEMPERATURE

A.-K. Lee | S.-R. Rhee | J.-K. Suh | H.-C. Cha

42. PROPAGATION AND PROTECTION OF FLOWER BULBS: CURRENT APPROACHES AND FUTURE PROSPECTS, WITH SPECIAL REFERENCE TO NARCISSUS

D.O. Sage

43. TECHNIQUES FOR THE IN VITRO PROPAGATION OF RHODOPHIALA AND LEUCOCORYNE SPP.

E. Olate | M. Bridgen

44. PROPAGATION OF BULBOUS ORNAMENTALS BY SIMPLE CULTURES OF BULB-SCALE SEGMENTS USING PLASTIC VESSELS

T. Yanagawa

45. PHOTOPERIOD AND STOCK PLANT AGE EFFECTS ON SHOOT, STOLON, AND RHIZOME FORMATION RESPONSE FROM LEAF CUTTINGS OF ACHIMENES

C.T. Miller | M. Bridgen

46. SENSITIVITY OF PATHOGEN INOCULA TO CHLORINE DIOXIDE GAS

G.A. Chastagner | K.L. Riley

47. THE DIFFERENTIATION AND THE DEVELOPMENT OF FLOWER BUDS DURING THE LIFE CYCLE OF PONERORCHIS GRAMINIFOLIA

S. Hamatani | Y. Niimi

48. EFFECT OF TEMPERATURE ON FLOWERING OF LACHENALIA RUBIDA (LILIACEAE)

E. Kodaira | S. Fukai

49. LLO HYBRID LILIES FROM L. x FORMOLONGI AND CHROMOSOME DOUBLED L. FORMOSANUM VAR. PRICEI x ORIENTAL HYBRID ‘LA REVE’

S. Fukai | T. Isobe | C. Ikoma

50. EFFECTS OF COLD TREATMENT ON ANTHESIS OF TULIP WITH SPECIAL REFERENCE TO POLLEN DEVELOPMENT

R.Y. Xu | Y. Niimi | D.-S. Han | S. Kuwayama

51. PRODUCTION OF HAPLOID AND DOUBLED HAPLOID PLANTS FROM ANTHER-DERIVED CALLUS OF LILIUM FORMOSANUM

D.-S. Han | Y. Niimi

T. Ishimori | Y. Niimi

53. AGROBACTERIUM-MEDIATED PRODUCTION OF TRANSGENIC PLANTS IN TRICYRTIS HIRTA(LILIACEAE)

Y. Adachi | S. Mori | M. Nakano

54. CROSS-COMPATIBILITY IN INTERSPECIFIC AND INTERGENERIC HYBRIDIZATION AMONG THE COLCHICACEOUS ORNAMENTALS, GLORIOSA SPP., LITTONIA MODESTA AND SANDERSONIA AURANTIACA

S. Kuwayama | Y. Mizuta | M. Nakano | T. Nakamura | T. Oomiya

55. ISOLATION AND CHARACTERIZATION OF A CYTOCHROME P450 GENE FROM MUSCARI ARMENIACUM

S. Mori | M. Nakano | M. Kondo | Y. Hoshi | H. Kobayashi

56. INHERITANCE OF FLOWER PIGMENT IN CROSSES BETWEEN CYCLAMEN CULTIVARS AND CYCLAMEN PURPURASCENS

T. Takamura | M. Aizawa | S.Y. Kim | M. Nakayama | H. Ishizaka

57. EFFECT OF NITROGEN AND POTASSIUM ON GROWTH AND DEVELOPMENT OF CURCUMA ALISMATIFOLIA GAGNEP.

58. MITOTIC AND MEIOTIC POLYPLOIDIZATION IN LILY HYBRIDS FOR TRANSFERRING BOTRYTIS RESISTANCE

C.M. Beers | R. Barba-Gonzalez | A.A. van Silfhout | M.S. Ramanna | J.M. van Tuyl

59. GENOTYPIC AND ENVIRONMENTAL VARIATION IN PRODUCTION OF 2N-GAMETES OF ORIENTAL x ASIATIC LILY HYBRIDS

A.C. Lokker | R. Barba-Gonzalez | K.-B. Lim | M.S. Ramanna | J.M. van Tuyl

60. DIFFERENCES IN EPICUTICULAR WAX LAYER IN TULIP CAN INFLUENCE RESISTANCE TO BOTRYTIS TULIPAE

A. Leon Reyes | T.P. Prins | J.-P. van Empel | J.M. van Tuyl

61. BIPARENTAL PLASTID INHERITANCE IN ZANTEDESHIA ALBOMACULATA (ARACEAE)

F.S. Brown | R.C. Snijder | J.M. van Tuyl

62. EPIDEMIOLOGICAL RESEARCH ON BOTRYTIS DISEASES OF TULIP PLANTS CAUSED BY B. TULIPAE AND B. CINEREA

63. INTERSPECIFIC HYBRIDIZATION OF WILD GLADIOLUS SPECIES USING A DENDROGRAM BASED RAPD ANALYSIS

64. CONANTHERA SPP. LIFE CYCLE AND ORNAMENTAL POTENTIAL

P. Yáñez | P. Peñailillo | F. Schiappacasse | K. Ohkawa

65. FLAVONOIDS AND THEIR DISTRIBUTION PATTERNS IN THE FLOWERS OF GLADIOLUS CULTIVARS

T. Takemura | Y. Takatsu | M. Kasumi | W. Marubashi | T. Iwashina

66. THE EFFECT OF CORM WEIGHT AND LOW TEMPERATURE TREATMENT ON THE FLOWERING OF ERYTHRONIUM PAGODA

K. Shinoda | N. Murata

67. ONLY FULL COOLING OF MOTHER TULIP BULBS IS NECESSARY FOR DAUGHTER BULB FORMATION FROM DAUGHTER BUDS

M. Saniewski | H. Okubo

68. EFFICACY OF FUNGICIDES IN CONTROLLING LEAF SPOT ON BULBOUS IRIS

G.A. Chastagner | P. Kaufmann

69. EFFECTS OF NUTRIENT SOLUTION MANAGEMENT AND METHODS OF STORAGE AND DISTRIBUTION ON FLOWERING AND QUALITY OF CUT IRIS, TULIP AND LILY

W.-H. Lee | J.-H. Kim | A.-K. Lee | J.-K. Suh | Y.-J. Yang

70. EFFECTS OF NUTRIENT SOLUTION COMPOSITION AND PLANT GROWTH RETARDANTS ON GROWTH AND FLOWERING IN HYDROPONICS OF CUT TULIP

K.H. Lee | J.-K. Suh

71. EFFECTS OF GIBBERELLIC ACID ON THE VASE LIFE OF CUT PATUMMA (CURCUMA ALISMATIFOLIA GAGNEP.) ‘CHAING MAI’ FLOWERS

T. Kjonboon | S. Kanlayanarat

72. EFFECT OF CUTTING METHOD ON BULB PRODUCTION OF HIPPEASTRUM HYBRIDUM IN TAIWAN

Y. Zhu | K.S. Liu | J.C. Yiu

73. THE INFLUENCE OF BA AND NAA ON ADVENTITIOUS BUD FORMATION IN LEAF CUTTINGS OF BEGONIA x TUBERHYBRIDA

Y. Shimada | G. Mori | Y. Katahara | M. Oda

74. AGROBACTERIUM-MEDIATED TRANSFORMATION OF LILIUM LONGIFLORUM

Y. Hoshi | M. Kondo | H. Kobayashi | S. Mori | M. Nakano

75. RESISTANCE OF TULIP CULTIVARS TO MILD MOTTLE MOSAIC DISEASE

T. Morikawa | Y. Taga | T. Morii

76. THE PRODUCTION AND CHARACTERISTICS OF THE NEW TULIP CULTIVAR, ‘ARISA’

77. A NEW CULTIVAR OF THE ASIATIC HYBRID LILY, ‘LI-9’, WITH MULTIPLE SCAPES AND SMALL FLOWERS

T. Oomiya | H. Tamagake | S. Tsutsui | M. Ubukata

78. CHANGES IN FREEZING TOLERANCE OF LILY BULBS OF FOUR HYBRID GROUPS DURING LONG-TERM STORAGE AT -2°C

H. Imanishi | H. Nagasako | K. Inamoto | M. Doi

79. EFFECT OF GROWING TEMPERATURES ON SCALY RHIZOME FORMATION OF ACHIMENES

Y. Koike | S. Suzuki

80. CHARACTERIZATION OF GENES RELATED TO HYACINTH BULB FORMATION INDUCED BY LOW TEMPERATURE OR ABA

K. Li-Nagasuga | H. Okubo

81. SEARCH FOR THE GENES REGULATING BULB FORMATION IN LILIUM SPECIOSUM BY DIFFERENTIAL DISPLAY

K. Maehara | K. Li-Nagasuga | H. Okubo

82. DORMANCY RELEASE OF EASTER LILY BULB BY LOW O₂ CONCENTRATION TREATMENTS

W.A. Wakakitsu Amaki | W.A. Wakanori Amaki

83. ANALYSIS OF FLOWER SCENT OF FREESIA SPECIES AND CULTIVARS

S. Wongchaochant | K. Inamoto | M. Doi

84. EFFECT OF LOW TEMPERATURE AND CHITOSAN ON DORMANCY BREAKING AND GROWTH OF YOUNG CORMS OF THREE ARISAEMA SPECIES

A. Hasegawa | R. Kanechika | S. Oguni

85. THE INFLUENCE OF NEW METHODS OF CORM COATING ON FREESIA GROWTH, DEVELOPMENT AND HEALTH

L. Startek | A. Bartkowiak | P. Salachna | M. Kaminska | K. Mazurkiewicz-Zapalowicz

86. EFFECT OF ETHEPHON ON EASY POT FREESIA

L. Startek | P. Zurawik

87. DISTANT HYBRIDS AND THEIR VERIFICATION BY MEANS OF RAPD

B. Ploszaj | S. Stojalowski | L. Startek | A. Zawadzinska

88. RAPID ENLARGEMENT OF LILY BULBLET BY BIOREACTOR CULTURE

D.H. Goo | J.H. Lim | H.R. Cho | Y.J. Kim | K.W. Kim

89. COMPARISON OF POLLEN MORPHOLOGY IN INTERSPECIFIC HYBRID LILIES AFTER IN VITRO CHROMOSOME DOUBLING

H.K. Rhee | H.R. Cho | K.J. Kim | K.S. Kim

90. LEVEL OF RESISTANCE TO FUSARIUM OXYSPORUM F. SP. LILII (FOL 4 AND 11) USING ENDOGENOUS ANTIFUNGAL SUBSTANCES EXTRACTED FROM BULBS AND/OR ROOTS IN LILIUM GENUS

J.H. Lim | S.T. Choi | H.K. Rhee | H.R. Cho | H.Y. Joung

91. VIRULENCE VARIATION IN 20 ISOLATES OF ERWINIA CAROTOVORA SUBSP. CAROTOVORA ON ZANTEDESCHIA CULTIVARS IN KOREA

92. CHANGES OF ENDOGENOUS GROWTH SUBSTANCES DURING BULB MATURATION AFTER FLOWERING IN LILIUM ORIENTAL HYBRID ‘CASA BLANCA’

K.J. Kim | K.S. Kim

93. FACTORS INFLUENCING MICROPROPAGATION OF NARCISSUS

D. Sochacki | T. Orlikowska

94. QUALITY EVALUATION OF FORCED TULIP FLOWERS DEPENDING ON BULB PRODUCTION ENVIRONMENT AND FORCING MEDIUM

D. Sochacki | E. Chojnowska

95. THE INFLUENCE OF GIBBERELLIC ACID ON GROWTH AND FLOWERING OF SOME ZANTEDESCHIA CULTIVARS GROWN OUTDOORS

J. Treder

96. INTERRUPTED COOLING AS AN ALTERNATIVE TO CASE-COOLED OR CONTROLLED TEMPERATURE FORCING OF EASTER LILIES

T.J. Blom | D. Kerec | R. Kamenetsky

97. CLONING OF THE HYMENOCALLIS SALISB.

H. Leszczynska-Borys | M.W. Borys | J.L. Galván

98. SOME CYTOLOGICAL OBSERVATIONS ON POLLEN GRAINS OF WILD POPPY ANEMONE VARIETIES FROM TURKEY

E. Ari | S. Buyukalaca | P. Gok | S. Eti

99. MICROPROPAGATION SCHEME OF CURCUMA ALISMATIFOLIA GAGNEP.

N. Toppoonyanont | S. Chongsang | S. Chujan | S. Somsueb | P. Nuamjaroen

100. ANATOMICAL AND HISTOCHEMICAL STUDIES OF THE ABSCISSION PROCESS IN THE JUNCTURE TISSUE BETWEEN THE PERIANTH AND RECEPTACLE TISSUE ON LILIUM ‘POLLYANNA’

T. Tabuchi | A. Uesugi | A. Furukawa | M. Moriyama | K. Toba

101. ANATOMICAL STUDIES OF THE LEAF YELLOWING PROCESS ON LILIUM ‘POLLYANNA’ FOLLOWING SOME PLANT GROWTH REGULATORS APPLICATION

T. Tabuchi | A. Uesugi | A. Furukawa | M. Moriyama | K. Toba

102. EFFECT OF GROWTH RETARDANTS ON FLOWER LONGEVITY AND THE ONSET OF LEAF YELLOWING AND NECROSIS ON GLORIOSA ‘FIRE BIRD’

T. Tabuchi | A. Furukawa | A. Uesugi | K. Toba

103. EFFECT OF PLANTING DEPTH AND DENSITY, LEAF REMOVAL, CYTOKININ AND GIBBERELLIC ACID TREATMENTS ON FLOWERING AND RHIZOME PRODUCTION IN ZANTEDESCHIA AETHIOPICA

G. Luria | D. Weiss | O. Ziv | A. Borochov

104. UTILIZATION OF EMBRYOGENIC CELL CULTURES FOR THE MASS PRODUCTION OF BULBLETS IN LILIES

S.K. Kim | B.J. Ahn

105. EFFECT OF RHIZOME STORAGE DURATION AND TEMPERATURE ON CARBOHYDRATE CONTENT, RESPIRATION, GROWTH, AND FLOWERING OF ORNAMENTAL GINGER

M. del Pilar Paz | J.S. Kuehny | G. McClure | C. Graham | R. Criley

106. A METHOD OF BULB YIELD PREDICTION IN TULIP

M. Amano | M. Kanamori | F. Imai

107. BULBOUS FLOWER PRODUCTION SYSTEMS: STATE OF THE ART

J. Wildschut | H. Gude | A. Krikke

108. EFFECT OF CALCIUM CONCENTRATIONS IN FERTILIZER SOLUTION ON GROWTH OF AND NURTRIENT UPTAKE BY ORIENTAL HYBRID LILY ‘CASA BLANCA’

J.M. Choi | K.H. Lee | E.M. Lee

109. EFFECT OF PHOSPHORUS CONCENTRATIONS IN FERTILIZER SOLUTION ON GROWTH OF AND NUTRIENT UPTAKE BY ORIENTAL HYBRID LILY ‘CASA BLANCA’

K.H. Lee | J.M. Choi

110. GROWING METHODS AND CHEMICAL DRENCHES CONTROL CALLA SOFT ROT

P.J. Wright | G.E. Clark | J. Koolaard