Articles
SEARCH FOR THE GENES REGULATING BULB FORMATION IN LILIUM SPECIOSUM BY DIFFERENTIAL DISPLAY
Article number
673_80
Pages
583 – 589
Language
English
Abstract
The purpose of this study is to clarify the relationship between bulb formation and the induction of bulb dormancy by identifying the genes expressed during bulb formation.
In vitro, explants of Lilium speciosum on MS medium formed bulbs at 25°C but not at 15°C. Bulb formation at 25°C was accelerated with ABA, whereas it was inhibited with fluridone.
Comparison of the gene expression in the explants under bulb-forming and non bulb-forming conditions (25°C vs. 15°C, 25°C vs. 25°C with fluridone and 25°C with ABA vs. 25°C with fluridone) was done by differential display analysis. cDNA was prepared from total RNA extracted from the scales at two-week interval from two to six weeks of culture, and differential display was performed with 50 primers.
The explants under bulb-forming conditions expressed 39 specific fragments, and those under non bulb-forming conditions did 42 in 32 primers.
Among 39 fragments, five were in common with the fragments found in hyacinth (Hyacinthus orientalis) cultured at 5°C (bulb-forming condition), and three of the five were also in common with those in hyacinth cultured both at 5°C and at 25°C with ABA (Ii-Nagasuga and Okubo, 2004). The fragments similarly expressed in Lilium speciosum cultured at 25°C, hyacinth at 5°C and hyacinth at 25°C with ABA, conditions for bulb formation, might be possibly the expressions of the genes which induce bulb formation.
In vitro, explants of Lilium speciosum on MS medium formed bulbs at 25°C but not at 15°C. Bulb formation at 25°C was accelerated with ABA, whereas it was inhibited with fluridone.
Comparison of the gene expression in the explants under bulb-forming and non bulb-forming conditions (25°C vs. 15°C, 25°C vs. 25°C with fluridone and 25°C with ABA vs. 25°C with fluridone) was done by differential display analysis. cDNA was prepared from total RNA extracted from the scales at two-week interval from two to six weeks of culture, and differential display was performed with 50 primers.
The explants under bulb-forming conditions expressed 39 specific fragments, and those under non bulb-forming conditions did 42 in 32 primers.
Among 39 fragments, five were in common with the fragments found in hyacinth (Hyacinthus orientalis) cultured at 5°C (bulb-forming condition), and three of the five were also in common with those in hyacinth cultured both at 5°C and at 25°C with ABA (Ii-Nagasuga and Okubo, 2004). The fragments similarly expressed in Lilium speciosum cultured at 25°C, hyacinth at 5°C and hyacinth at 25°C with ABA, conditions for bulb formation, might be possibly the expressions of the genes which induce bulb formation.
Publication
Authors
K. Maehara, K. Li-Nagasuga, H. Okubo
Keywords
bulb dormancy, abscisic acid, fluridone, hyacinth, in vitro
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