Articles
MICROPROPAGATION SCHEME OF CURCUMA ALISMATIFOLIA GAGNEP.
Article number
673_98
Pages
705 – 712
Language
English
Abstract
This study was conducted to develop efficient and repeatable micropropagation scheme for Curcuma alismatifolia Gagnep., starting from initiation, multiplication, to rooting stage.
During the initiation stage, Curcuma alismatifolia Gagnep. coflorescences were removed from each pouch of inflorescence, and used as starting materials.
They were cultured in a modified MS (1962) containing 10 mg/L BA and 0.1 mg/L IAA. After 1 month in culture, they developed and reverted to vegetative shoots located at the same positions and arranged spirally within the bracteole, similarly to those in vivo.
It was clear from anatomical analysis that shoots directly emerged from flower organs, not via callus formation.
During multiplication stage factors such as BA and TDZ were evaluated at concentrations of 0, 0.1 and 0.5 mg/L with combinations of Imazalil (IMA) at 0, 2 and 4 mg/L. It was found that 0.5 mg/L TDZ in combination of 4 mg/L IMA was able to increase 30-40 new shoots from one explant with retarded shoot morphology.
Before transferring to the greenhouse these retarded shoots elongated and rooted in medium containing 0.3 mg/L BA and 0.1 mg/L IAA, and 0.1 mg/L IAA, respectively.
The micropropagation scheme is also presented.
During the initiation stage, Curcuma alismatifolia Gagnep. coflorescences were removed from each pouch of inflorescence, and used as starting materials.
They were cultured in a modified MS (1962) containing 10 mg/L BA and 0.1 mg/L IAA. After 1 month in culture, they developed and reverted to vegetative shoots located at the same positions and arranged spirally within the bracteole, similarly to those in vivo.
It was clear from anatomical analysis that shoots directly emerged from flower organs, not via callus formation.
During multiplication stage factors such as BA and TDZ were evaluated at concentrations of 0, 0.1 and 0.5 mg/L with combinations of Imazalil (IMA) at 0, 2 and 4 mg/L. It was found that 0.5 mg/L TDZ in combination of 4 mg/L IMA was able to increase 30-40 new shoots from one explant with retarded shoot morphology.
Before transferring to the greenhouse these retarded shoots elongated and rooted in medium containing 0.3 mg/L BA and 0.1 mg/L IAA, and 0.1 mg/L IAA, respectively.
The micropropagation scheme is also presented.
Publication
Authors
N. Toppoonyanont, S. Chongsang, S. Chujan, S. Somsueb, P. Nuamjaroen
Keywords
Curcuma alismatifolia Gagnep., thidiazuron, imazalil, retarded shoot, micropropagation
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