Articles
THE INFLUENCE OF BA AND NAA ON ADVENTITIOUS BUD FORMATION IN LEAF CUTTINGS OF BEGONIA x TUBERHYBRIDA
Article number
673_72
Pages
537 – 541
Language
English
Abstract
Begonia x tuberhybrida is propagated mainly by seed, because vegetative propagation by cuttings or tissue culture is difficult.
However, seedlings generally exhibit a wide range of variation due to their high level of heterozygosity, and also the pollination period is limited.
An adequate technique of vegetative propagation of B. x tuberhybrida if established, would be useful for commercial production.
Therefore, we investigated the propagation characteristics of the stem and leaf cuttings, and influence of BA and NAA on adventitious bud formation in leaf cuttings of B. x tuberhybrida.
Stems (2 cm in length) with 5 cm of basal part of leaf blades but with axillary buds removed, and leaf pieces (2.5 x 2.5 cm) cut from young expanded leaves were planted in a mixture of vermiculite and perlite on a bench in a glasshouse controlled at 25/17°C (DT/NT). Both of the stems and the leaf pieces formed only roots or calli, but none of them formed any adventitious buds.
Effects of plant growth regulators on adventitious bud formation were examined by planting leaf pieces (2 x 1.5 cm) on agar medium containing NAA (0-0.5 or 1 ppm) and/or BA (0, 0.5 or 1 ppm) at 23°C. When leaf pieces were planted on agar medium without plant growth regulators, the leaf piece did not form adventitious buds.
On the medium with BA, adventitious buds were formed.
The leaf pieces planted on the medium with 1 ppm BA formed adventitious buds in 25% and callus in 31% of them.
Most leaf pieces in the medium containing NAA died.
BA may be effective for inducing adventitious bud formation in leaf cuttings of B. x tuberhybrida.
However, seedlings generally exhibit a wide range of variation due to their high level of heterozygosity, and also the pollination period is limited.
An adequate technique of vegetative propagation of B. x tuberhybrida if established, would be useful for commercial production.
Therefore, we investigated the propagation characteristics of the stem and leaf cuttings, and influence of BA and NAA on adventitious bud formation in leaf cuttings of B. x tuberhybrida.
Stems (2 cm in length) with 5 cm of basal part of leaf blades but with axillary buds removed, and leaf pieces (2.5 x 2.5 cm) cut from young expanded leaves were planted in a mixture of vermiculite and perlite on a bench in a glasshouse controlled at 25/17°C (DT/NT). Both of the stems and the leaf pieces formed only roots or calli, but none of them formed any adventitious buds.
Effects of plant growth regulators on adventitious bud formation were examined by planting leaf pieces (2 x 1.5 cm) on agar medium containing NAA (0-0.5 or 1 ppm) and/or BA (0, 0.5 or 1 ppm) at 23°C. When leaf pieces were planted on agar medium without plant growth regulators, the leaf piece did not form adventitious buds.
On the medium with BA, adventitious buds were formed.
The leaf pieces planted on the medium with 1 ppm BA formed adventitious buds in 25% and callus in 31% of them.
Most leaf pieces in the medium containing NAA died.
BA may be effective for inducing adventitious bud formation in leaf cuttings of B. x tuberhybrida.
Publication
Authors
Y. Shimada, G. Mori, Y. Katahara, M. Oda
Keywords
hybrid tuberous begonia, propagation, plant growth regulators, vegetative propagation
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