Articles
FACTORS INFLUENCING MICROPROPAGATION OF NARCISSUS
Article number
673_92
Pages
669 – 673
Language
English
Abstract
The aim of the experiments was to improve the micropropagation protocol for narcissus (Narcissus L.) in three respects to achieve: a decrease in contamination of initial explants, an increase in regeneration and multiplication efficiency, and shortening of the bulbil formation period.
For the cultivar Carlton, a temperature treatment of bulbs (44.4°C for 3 h) in hot water and for Heweliusz hot 1% aqueous solution of imazalil (Fungaflor 200), followed in either case by sterilization of bulb segments in 0.1% mercuric chloride and 0.75% chloramine T, resulted in a reduction of the initial contamination to the level of 14 and 17%, respectively.
Prolongation of the regeneration stage up to 24 weeks significantly increased the number of regenerated shoots but the most intensive regeneration period depended on the genotype.
An increase in the multiplication rate was achieved by cutting bulbils into four parts.
The number of shoots varied from 9.2 to 13.1 shoots per 1/4 of bulbil, depending on the concentration of growth regulators.
The duration of the bulbil formation period was shortened by 4 weeks by reducing incubation time from eight to four weeks at 20/25°C and increasing the cooling temperature from 5 to 8°C.
For the cultivar Carlton, a temperature treatment of bulbs (44.4°C for 3 h) in hot water and for Heweliusz hot 1% aqueous solution of imazalil (Fungaflor 200), followed in either case by sterilization of bulb segments in 0.1% mercuric chloride and 0.75% chloramine T, resulted in a reduction of the initial contamination to the level of 14 and 17%, respectively.
Prolongation of the regeneration stage up to 24 weeks significantly increased the number of regenerated shoots but the most intensive regeneration period depended on the genotype.
An increase in the multiplication rate was achieved by cutting bulbils into four parts.
The number of shoots varied from 9.2 to 13.1 shoots per 1/4 of bulbil, depending on the concentration of growth regulators.
The duration of the bulbil formation period was shortened by 4 weeks by reducing incubation time from eight to four weeks at 20/25°C and increasing the cooling temperature from 5 to 8°C.
Publication
Authors
D. Sochacki, T. Orlikowska
Keywords
Narcissus L., contamination, regeneration, multiplication efficiency, bulbil formation
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