Articles
CROSS-COMPATIBILITY IN INTERSPECIFIC AND INTERGENERIC HYBRIDIZATION AMONG THE COLCHICACEOUS ORNAMENTALS, GLORIOSA SPP., LITTONIA MODESTA AND SANDERSONIA AURANTIACA
Article number
673_53
Pages
421 – 427
Language
English
Abstract
Interspecific and intergeneric cross-pollination and subsequent ovule culture were carried out among 6 genotypes of Gloriosa spp., 1 genotype of L. modesta and 2 genotypes of S. aurantiaca in order to widen their variations and to develop novel cultivars.
Ovules with placental tissues prepared from ovaries 14 days after pollination were cultured at 25°C in the dark on a medium containing 0.01 mg l-1
-naphthaleneacetic acid (NAA) and 0.01 mg l-1 benzyladenine (BA), on which rhizome-like structures were produced from the ovules in 49 out of 61 cross-combinations.
Following transfer to a medium containing 0.25 mg l-1 NAA and 2.5 mg l-1 BA under a 16-h photoperiod, some of the rhizome-like structures produced shoots and/or root-like structures.
These shoots developed into plantlets and produced tubers on a plant growth regulator-free medium.
In some of the plantlets derived from intergeneric cross-pollination, early confirmation of the hybridity was accomplished by flow cytometry analysis of relative DNA content of nuclei.
Ovules with placental tissues prepared from ovaries 14 days after pollination were cultured at 25°C in the dark on a medium containing 0.01 mg l-1
-naphthaleneacetic acid (NAA) and 0.01 mg l-1 benzyladenine (BA), on which rhizome-like structures were produced from the ovules in 49 out of 61 cross-combinations.Following transfer to a medium containing 0.25 mg l-1 NAA and 2.5 mg l-1 BA under a 16-h photoperiod, some of the rhizome-like structures produced shoots and/or root-like structures.
These shoots developed into plantlets and produced tubers on a plant growth regulator-free medium.
In some of the plantlets derived from intergeneric cross-pollination, early confirmation of the hybridity was accomplished by flow cytometry analysis of relative DNA content of nuclei.
Publication
Authors
S. Kuwayama, Y. Mizuta, M. Nakano, T. Nakamura, T. Oomiya
Keywords
cross-pollination, FCM analysis, ovule culture, rhizome-like structures, wide hybridization
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