SOME CYTOLOGICAL OBSERVATIONS ON POLLEN GRAINS OF WILD POPPY ANEMONE VARIETIES FROM TURKEY

There are four native varieties of poppy anemone, namely, Anemone coronaria var. coccinea (red flowered), Anemone coronaria var. alba (white flowered) Anemone coronaria var. rosea (pink flowered) and Anemone coronaria var. cyanea (purple flowered) in Turkey.
In this study, flowers of the four varieties were used as plant material in order to determine viability, germination rate and production of the pollen grains.
For the determination of pollen viability, TTC (Triphenyl tetrazolium chloride) and FDA (Fluorescein diacetate) were used.
In vitro pollen germination was performed using an agar method and pollen production was counted with the hemacytometric lam method.
The maximum pollen viability rate was obtained from the violet-flowered variety in TTC whilst the difference was not found to be statistically important among the varieties when FDA was used.
However, viability values of FDA were higher than that of TTC. As far as the pollen germination was concerned, the maximum germination was observed from the red-flowered variety in a medium with 1% agar + 25% sucrose + 150 ppm H₃BO₃. Additionally, the average number of anthers, the pollen production per anther and per flower, morphological homogeneity level and dimensions of pollen were determined for each variety.

IX International Symposium on Flower Bulbs

E. Ari, S. Buyukalaca, P. Gok, S. Eti

Anemone coronaria, poppy anemone, pollen viability, pollen germination, pollen production

https://doi.org/10.17660/ActaHortic.2005.673.97