Articles
MICROPROPAGATION OF GUAVA (PSIDIUM GUAJAVA L.)
Article number
735_20
Pages
155 – 158
Language
English
Abstract
A foolproof method of micropropagation through in vitro shoot bud culture has been developed in guava cv. Allahabad Safeda. The stock plant of guava was maintained at shade net house (75% shade) and pruned severely to encourage new growth.
Around 3 cm long shoots were excised and defoliated.
The explants were pretreated in solution containing 0.1% Carbendazime and 100 mg/L PVP for 1 hour and then washed with Tween-20 wetting agent.
The pretreated explants were further treated with HgCl2 0.1% for 5 minutes aseptically followed by six washing in autoclaved distilled water.
The sterilized explants were cultured on MS medium supplemented with BAP (3.0 mg/L) for shoot bud induction and proliferation.
The proliferated shoots were then subcultured on MS medium containing 10 mg/L IBA for rhizogenesis.
The rooted plants were finally shifted to autoclaved coconut husk fortified with ½ MS salt mixture for acclimatization.
Acclimatized plantlets of guava cv.
Allahabad Safeda were planted.
Around 3 cm long shoots were excised and defoliated.
The explants were pretreated in solution containing 0.1% Carbendazime and 100 mg/L PVP for 1 hour and then washed with Tween-20 wetting agent.
The pretreated explants were further treated with HgCl2 0.1% for 5 minutes aseptically followed by six washing in autoclaved distilled water.
The sterilized explants were cultured on MS medium supplemented with BAP (3.0 mg/L) for shoot bud induction and proliferation.
The proliferated shoots were then subcultured on MS medium containing 10 mg/L IBA for rhizogenesis.
The rooted plants were finally shifted to autoclaved coconut husk fortified with ½ MS salt mixture for acclimatization.
Acclimatized plantlets of guava cv.
Allahabad Safeda were planted.
Publication
Authors
M. Mishra, R. Chandra, R. Pati, A. Bajpai
Keywords
shoot bud culture, proliferation, MS media
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