Articles
ASSESSMENT OF GENETIC DIVERSITY AND RELATIONSHIP AMONG PSIDIUM SPP. THROUGH RAPD ANALYSIS
Article number
735_7
Pages
71 – 78
Language
English
Abstract
This investigation was aimed to examine the diversity among twenty-two Psidium guajava L. cultivars and two species viz.
P. catteilianum and P. friedrichthalianum using RAPD markers.
High molecular weight genomic DNA was isolated by the modified CTAB method.
Genetic variation was studied using forty-one random decamer primers, thirty-nine of that were reproducible.
For the various genotypes tested, between 2-16 bands were obtained for each primer.
Out of total 376 clear and reproducible bands, 347 were polymorphic accounting to 92.29% polymorphism.
The amplified DNA fragment normally ranged from 0.12-2.2 kilo base pair.
The primers screened revealed RAPD fragment(s) unique to a particular genotype.
PIC value was found to be maximum for primer OPB-12 (0.916) and least for OPD-11 (0.520). A genetic similarity matrix was constructed and the values ranged between 0.33-0.94. The highest genetic similarity (0.94) was noticed between Hybrid Red Supreme and Super Max Ruby, whereas Hybrid Red Supreme and Spear Acid were found to be genetically most diverse (0.33). In the dendrogram constructed by NTSYS-PC, all the 22 genotypes were grouped under two clusters.
In the first cluster Chinese guava grouped together with P. guajava L. cultivar Apple Colour and Spear Acid and the second cluster comprised of the remaining 19 genotypes.
The study thus demonstrated the usefulness of the RAPD markers in effectively assessing the genetic relationship present among the various genotypes of Psidium spp. examined.
P. catteilianum and P. friedrichthalianum using RAPD markers.
High molecular weight genomic DNA was isolated by the modified CTAB method.
Genetic variation was studied using forty-one random decamer primers, thirty-nine of that were reproducible.
For the various genotypes tested, between 2-16 bands were obtained for each primer.
Out of total 376 clear and reproducible bands, 347 were polymorphic accounting to 92.29% polymorphism.
The amplified DNA fragment normally ranged from 0.12-2.2 kilo base pair.
The primers screened revealed RAPD fragment(s) unique to a particular genotype.
PIC value was found to be maximum for primer OPB-12 (0.916) and least for OPD-11 (0.520). A genetic similarity matrix was constructed and the values ranged between 0.33-0.94. The highest genetic similarity (0.94) was noticed between Hybrid Red Supreme and Super Max Ruby, whereas Hybrid Red Supreme and Spear Acid were found to be genetically most diverse (0.33). In the dendrogram constructed by NTSYS-PC, all the 22 genotypes were grouped under two clusters.
In the first cluster Chinese guava grouped together with P. guajava L. cultivar Apple Colour and Spear Acid and the second cluster comprised of the remaining 19 genotypes.
The study thus demonstrated the usefulness of the RAPD markers in effectively assessing the genetic relationship present among the various genotypes of Psidium spp. examined.
Publication
Authors
A.S. Sharma, S.K. Sehrawat, R.S. Singhrot, K.S. Boora
Keywords
Psidium spp., RAPD markers, polymorphism, genetic relationship
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