Articles
MOLECULAR CHARACTERIZATION OF INTERSPECIFIC HYBRIDS OF SCENTED ROSES USING RAPD MARKERS
Article number
751_20
Pages
175 – 179
Language
English
Abstract
Rosa damascena Mill. (Family: Rosaceae) is a popular scented rose of value in the essential oil industry.
The flowering period is limited to 4-6 weeks each year depending upon the weather conditions.
Two cultivars, namely Jwala and Himroz, of R. damascena were crossed with R. bourboniana Desp. with a perpetual flowering habit in a reciprocal manner and hybrids were obtained and characterized by RAPD analysis.
Eight hybrids were from a cross of R. damascena × R. bourboniana and four hybrids from R. bourboniana × R. damascena. RAPD analysis was done across three parental lines screening 180 primers of operon series (OPA, OPE, OPF, OPG, OPC, OPM, OPO, OPS, OPV). A total of 58 primers were found to be evincing polymorphic bands among the parents.
However, only 24 of these primers amplified male parent specific fragments that were utilized for distinguishing 12 hybrids.
Cluster analysis based on Jaccards coefficient using UPGMA showed considerable genetic variation in the 12 hybrids.
All the 12 hybrids were clustered into three different groups.
The average similarity index across the hybrids was 0.63. Since the parental genotypes were selections from an open pollinated population and propagated clonally, they were highly heterozygous.
Hence, recombination gives rise to variations at the gametic level and the hybrids cannot be true to type or uniform like F1 plants.
However, considering the interspecific nature of the cross, the hybrids offer an opportunity to recombine complementary traits of high oil content and extended flowering duration.
The flowering period is limited to 4-6 weeks each year depending upon the weather conditions.
Two cultivars, namely Jwala and Himroz, of R. damascena were crossed with R. bourboniana Desp. with a perpetual flowering habit in a reciprocal manner and hybrids were obtained and characterized by RAPD analysis.
Eight hybrids were from a cross of R. damascena × R. bourboniana and four hybrids from R. bourboniana × R. damascena. RAPD analysis was done across three parental lines screening 180 primers of operon series (OPA, OPE, OPF, OPG, OPC, OPM, OPO, OPS, OPV). A total of 58 primers were found to be evincing polymorphic bands among the parents.
However, only 24 of these primers amplified male parent specific fragments that were utilized for distinguishing 12 hybrids.
Cluster analysis based on Jaccards coefficient using UPGMA showed considerable genetic variation in the 12 hybrids.
All the 12 hybrids were clustered into three different groups.
The average similarity index across the hybrids was 0.63. Since the parental genotypes were selections from an open pollinated population and propagated clonally, they were highly heterozygous.
Hence, recombination gives rise to variations at the gametic level and the hybrids cannot be true to type or uniform like F1 plants.
However, considering the interspecific nature of the cross, the hybrids offer an opportunity to recombine complementary traits of high oil content and extended flowering duration.
Authors
N. Kaur, R.K. Sharma, D. Dhyani, S. Karthigeyan, P.S. Ahuja
Keywords
RAPD, genetic diversity, R. damascena, R. bourboniana
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