Articles
ELIMINATION OF ROSE MOSAIC VIRUSES USING MICROSHOOT TIP TISSUE CULTURE
Article number
751_29
Pages
237 – 239
Language
English
Abstract
The effect of microshoot tip culture on virus elimination in roses infected with Apple mosaic virus (ApMV) and Prunus necrotic ringspot virus (PNRSV) was studied.
Microshoot tips, approximately 0.2 0.8 mm, consisting of a meristematic dome plus a few leaf primordia, were aseptically excised from an actively growing plant, established on tissue culture medium, and grown into a new mother plant.
Candidate mother plants were tested for virus by enzyme-linked immunosorbent assay (ELISA) and biological indexing on Rosa multiflora Burr and Prunus serrulata Lindl. Shirofugen. Percent survival and rooting in tissue culture was low and variety dependent.
Of 12 varieties treated with tissue culture therapy, plants were regenerated from the following six: Gold Glow, Curly Pink, Lily Pons, Queen of the Lakes, 6018 Climber, and Elina. Testing began two years ago and is ongoing.
Virus testing to date shows that 41/57 or 72% of the plants tested negative for viruses.
Microshoot tips, approximately 0.2 0.8 mm, consisting of a meristematic dome plus a few leaf primordia, were aseptically excised from an actively growing plant, established on tissue culture medium, and grown into a new mother plant.
Candidate mother plants were tested for virus by enzyme-linked immunosorbent assay (ELISA) and biological indexing on Rosa multiflora Burr and Prunus serrulata Lindl. Shirofugen. Percent survival and rooting in tissue culture was low and variety dependent.
Of 12 varieties treated with tissue culture therapy, plants were regenerated from the following six: Gold Glow, Curly Pink, Lily Pons, Queen of the Lakes, 6018 Climber, and Elina. Testing began two years ago and is ongoing.
Virus testing to date shows that 41/57 or 72% of the plants tested negative for viruses.
Authors
D.A. Golino, S.T. Sim, J.A. Lee, A. Rowhani
Keywords
Apple mosaic virus, Prunus necrotic ringspot virus
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