Articles
MICROPROPAGATION OF ROSA × HYBRIDA CV. ‘VEGAS’ VIA AXILLARY BUDS
Article number
751_52
Pages
407 – 411
Language
English
Abstract
The world’s leading cut flower is rose.
Growers propagate roses by cutting or grafting.
All these methods are efficient but spread diseases.
Micropropagation is a tool that allows solving this problem.
In this study culture medias were tested in order to establish, multiply and root Rosa × hybrida cv. Vegas in vitro via axillary buds.
The best results for micropropagation were obtained with buds containing 5 to 7 primordia developed in MS (Murashige and Skoog, 1962) culture media supplemented with 0.5 mg.L-1 BAP + 0.25 mg.L-1 GA3 + 0.01 mg.L-1 IAA, multiplied in MS media supplemented with 1.0 mg.L-1 BAP + 0.1 mg.L-1 GA3 and rooted in ½MS without growth regulators.
Growers propagate roses by cutting or grafting.
All these methods are efficient but spread diseases.
Micropropagation is a tool that allows solving this problem.
In this study culture medias were tested in order to establish, multiply and root Rosa × hybrida cv. Vegas in vitro via axillary buds.
The best results for micropropagation were obtained with buds containing 5 to 7 primordia developed in MS (Murashige and Skoog, 1962) culture media supplemented with 0.5 mg.L-1 BAP + 0.25 mg.L-1 GA3 + 0.01 mg.L-1 IAA, multiplied in MS media supplemented with 1.0 mg.L-1 BAP + 0.1 mg.L-1 GA3 and rooted in ½MS without growth regulators.
Authors
A. Drefahl, M.G. Quoirin, F. Lorena Cuquel
Keywords
floriculture, cut flower, meristem, rose, tissue culture
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