Articles
TETRAPLOID INDUCTION BY COLCHICINE AND ORYZALIN IN ROSA MULTIFLORA
Article number
751_40
Pages
313 – 322
Language
English
Abstract
Rosa hybrida PEKcougel, which was tetraploid, was selected for resistance against crown gall disease, and R. multiflora, which was diploid, had resistance against root rot disease.
A variety of rootstock with resistance to both crown gall and root rot diseases was not able to be bred by cross pollination between PEKcougel and R. multiflora. So, we attempted to induce tetraploidy of R. multiflora by colchicine and oryzalin and cross-pollinate between PEKcougel and R. multiflora. Germinated seeds of R. multiflora with root lengths of 12 mm were dipped into colchicine and oryzalin solution and planted to pots.
Sprouting and survival were inhibited by colchicine and oryzalin.
The plantlets with over five leaves were tested for tetraploid analysis by flow cytometry analysis.
Six chimeras and two tetraploids were induced by 3.0 x 10-4 M and 3.0 x 10-3 M colchicine.
The leaflets of diploids were lanceolate-oblong, and those of chimeras and tetraploids were oval.
The guard cells of tetraploids were obviously larger than those of diploids and chimeras, but the chimeras could not be distinguished from diploids in terms of the length of guard cells.
The flower of tetraploids was larger than that of diploids.
As tetraploids had fertility, we obtained many hybrids with PEKcougel.
A variety of rootstock with resistance to both crown gall and root rot diseases was not able to be bred by cross pollination between PEKcougel and R. multiflora. So, we attempted to induce tetraploidy of R. multiflora by colchicine and oryzalin and cross-pollinate between PEKcougel and R. multiflora. Germinated seeds of R. multiflora with root lengths of 12 mm were dipped into colchicine and oryzalin solution and planted to pots.
Sprouting and survival were inhibited by colchicine and oryzalin.
The plantlets with over five leaves were tested for tetraploid analysis by flow cytometry analysis.
Six chimeras and two tetraploids were induced by 3.0 x 10-4 M and 3.0 x 10-3 M colchicine.
The leaflets of diploids were lanceolate-oblong, and those of chimeras and tetraploids were oval.
The guard cells of tetraploids were obviously larger than those of diploids and chimeras, but the chimeras could not be distinguished from diploids in terms of the length of guard cells.
The flower of tetraploids was larger than that of diploids.
As tetraploids had fertility, we obtained many hybrids with PEKcougel.
Authors
H. Fukui, T. Yokota
Keywords
crown gall, root rot, resistance, PEKcougel, chimera
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