Articles
INTRODUCTION OF WILD GERMPLASM IN MODERN ROSES
Article number
751_36
Pages
285 – 290
Language
English
Abstract
Introduction of germplasm from wild species is a prerequisite for the enlargement of the relatively small gene pool in roses.
In the present study testcrosses between different European wild species and selected tetraploid cultivated roses were made to evaluate crossing efficiencies.
Significant differences were observed among the tested combinations.
Interspecific crosses frequently generate F1 plants with odd chromosome numbers (mostly triploids). These triploids produce both monoploid and diploid pollen fractions.
Pollen sorting could increase the percentage of diploid pollen before it is used for pollination.
To evaluate the genetic transfer between wild and cultivated roses a protocol for making DNA microsatellites profiles of a single pollen was developed.
In the present study testcrosses between different European wild species and selected tetraploid cultivated roses were made to evaluate crossing efficiencies.
Significant differences were observed among the tested combinations.
Interspecific crosses frequently generate F1 plants with odd chromosome numbers (mostly triploids). These triploids produce both monoploid and diploid pollen fractions.
Pollen sorting could increase the percentage of diploid pollen before it is used for pollination.
To evaluate the genetic transfer between wild and cultivated roses a protocol for making DNA microsatellites profiles of a single pollen was developed.
Authors
J. Van Huylenbroeck, T. Eeckhaut, L. Leus, G. Werlemark, J. De Riek
Keywords
Rosa, breeding, polyploidy, flow cytometry, interspecific hybridisation, pollen sorting
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