Articles
SOMATIC EMBRYOGENESIS IN CARICA PAPAYA THROUGH ZYGOTIC EMBRYO DERIVED CALLUS CULTURE
Article number
851_29
Pages
201 – 208
Language
English
Abstract
A rapid and efficient system for papaya (Carica papaya L. cv.
Co7) regeneration through indirect somatic embryogenesis has been established.
Embryogenic calli were obtained from zygotic embryos (excised from 110-120 day-old fruits) on CIM [half-strength MS (Murashige and Skoog, 1962) basal salts and vitamins, 400 mg/L of glutamine, 6% sucrose, 4 g/L phytagel] supplemented with varying concentrations 0.5-12.0 mg/L of 2,4-D. The frequency of somatic embryo formation was as high as 54.35% when cultured on medium containing 2.0 mg/L of 2,4-D. Suspension cultures were superior to static cultures (solid medium) for the maturation of somatic embryos.
Matured (cotyledonary stage) somatic embryos cultured on MSR medium supplemented with 0.4 mg/L BAP and 0.02 mg/L NAA showed regeneration efficiency up to 62.32%. The emerging shoots with 2-3 trilobed leaves were rooted on MSL medium containing 1.0 mg/L of IBA with efficiency of 60.07%. Plants with well-developed shoots and roots were subsequently hardened on a potting mixture enriched with arbuscular mycorrhiza.
The plantlets from zygotic embryo explants were morphologically similar and normal.
No phenotypic variations have been observed in field-established plants.
This regeneration protocol assures a high frequency of somatic embryogenesis, maturation and regeneration into plantlets.
Co7) regeneration through indirect somatic embryogenesis has been established.
Embryogenic calli were obtained from zygotic embryos (excised from 110-120 day-old fruits) on CIM [half-strength MS (Murashige and Skoog, 1962) basal salts and vitamins, 400 mg/L of glutamine, 6% sucrose, 4 g/L phytagel] supplemented with varying concentrations 0.5-12.0 mg/L of 2,4-D. The frequency of somatic embryo formation was as high as 54.35% when cultured on medium containing 2.0 mg/L of 2,4-D. Suspension cultures were superior to static cultures (solid medium) for the maturation of somatic embryos.
Matured (cotyledonary stage) somatic embryos cultured on MSR medium supplemented with 0.4 mg/L BAP and 0.02 mg/L NAA showed regeneration efficiency up to 62.32%. The emerging shoots with 2-3 trilobed leaves were rooted on MSL medium containing 1.0 mg/L of IBA with efficiency of 60.07%. Plants with well-developed shoots and roots were subsequently hardened on a potting mixture enriched with arbuscular mycorrhiza.
The plantlets from zygotic embryo explants were morphologically similar and normal.
No phenotypic variations have been observed in field-established plants.
This regeneration protocol assures a high frequency of somatic embryogenesis, maturation and regeneration into plantlets.
Publication
Authors
R. Anandan, Phan Dinh Phap, K. Soorianathasundaram, N. Kumar, S. Thirugnanakumar, D. Sudhakar, P. Balasubramanian
Keywords
papaya, somatic embryos, zygotic embryos
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