Articles
PHYLOGENETIC ANALYSIS IN THE GENERA PHAIUS AND CEPHALANTHEROPSIS USING RPL32-TRNL MARKER
Article number
878_11
Pages
107 – 113
Language
English
Abstract
The taxonomic status of Cephalantheropsis gracilis and Cephalantheropsis calanthoides remained uncertain before 1970, either pertained to the genus Phaius or Calanthe. Four species in the genus Phaius, two species in the genus Cephalantheropsis, and three species in the genus Calanthe endemic to Taiwan, were analyzed with rpl32-trnL marker to assess their phylogenetic relationship.
A dendrogram based on sequence analysis of rpl32-trnL marker showed that Phaius tankervilleae, Phaius flavus, Phaius takeoi and Phaius mishmensis were clustered together and Cephalantheropsis gracilis was grouped with Cephalantheropsis calanthoides.
Calanthe alismifolia, Calanthe sieboldii and Calanthe triplicata were clustered together as the outgroup.
The present results strongly support the traditional taxonomy as described by Hu (1972) and Liu and Su (1978). The rpl32-trnL marker which is a non-coding sequence of chloroplast DNA is a useful genetic marker for phylogenetic analysis between species.
A dendrogram based on sequence analysis of rpl32-trnL marker showed that Phaius tankervilleae, Phaius flavus, Phaius takeoi and Phaius mishmensis were clustered together and Cephalantheropsis gracilis was grouped with Cephalantheropsis calanthoides.
Calanthe alismifolia, Calanthe sieboldii and Calanthe triplicata were clustered together as the outgroup.
The present results strongly support the traditional taxonomy as described by Hu (1972) and Liu and Su (1978). The rpl32-trnL marker which is a non-coding sequence of chloroplast DNA is a useful genetic marker for phylogenetic analysis between species.
Publication
Authors
C.-L. Chen, F.-S. Liao, S.-H. Cheng
Keywords
Cephalantheropsis gracilis, Cephalantheropsis calanthoides, Calanthe, dendrogram
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