Articles
CHITOSAN STIMULATES GROWTH OF MICROPROPAGATED PLANTLETS
Article number
878_24
Pages
205 – 212
Language
English
Abstract
The effect of chitosans on growth of Dendrobium AW 179 plantlets in vitro was evaluated at 0, 50, 250 or 1,250 mg/L in modified Vacin and Went (VW) medium.
It was found that 50 mg/L chitosan significantly enhanced plantlet growth.
The number of shoots, leaves or roots of plantlets increased 2.3, 3.6 and 1.8 fold, respectively, compared to the medium without chitosan after 12 weeks of culture.
However, no stimulatory effect of chitosan was observed at higher concentrations.
Supplementation of triacontanol (TRIA) at 2 mg/L to the medium containing 50 mg/L chitosan led to a reduction in fresh weight, shoot diameter and leaf width, but an increase in leaf length of plantlets in comparison with the chitosan medium.
It was found that 50 mg/L chitosan significantly enhanced plantlet growth.
The number of shoots, leaves or roots of plantlets increased 2.3, 3.6 and 1.8 fold, respectively, compared to the medium without chitosan after 12 weeks of culture.
However, no stimulatory effect of chitosan was observed at higher concentrations.
Supplementation of triacontanol (TRIA) at 2 mg/L to the medium containing 50 mg/L chitosan led to a reduction in fresh weight, shoot diameter and leaf width, but an increase in leaf length of plantlets in comparison with the chitosan medium.
Publication
Authors
P. Tantasawat, A. Wannajindaporn, C. Chantawaree, C. Wangpunga, K. Poomsom, A. Sorntip
Keywords
melissyl alcohol, micropropagation, tissue culture, triacontanol
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