EFFECTS OF CULTURE MEDIA AND CAPSULE MATURITY ON SEED GERMINATION OF INTRASPECIFIC AND INTERSPECIFIC CROSSES IN THE GENUS CALANTHE

In order to overcome the low percentage of seed germination in hybridization within and between species in the genus Calanthe, intraspecific crosses (sib crosses) of C. triplicata and C. masuca and interspecific crosses of C. triplicata × C. masuca, and its reciprocal were made.
Seeds at different capsule maturity stages were harvested and cultured on Hyponex #1-based media with different additives.
In intraspecific crosses, the highest percentage of seed germination in C. triplicata was 25.7% for seeds from capsules harvested on 188 days after pollination and cultured on basal medium, while that in C. masuca was 28.5% for seeds from capsules harvested 91 days after pollination and cultured on basal medium + 1 mg/L kinetin.
In interspecific crosses, the highest seed germination percentage was 9.3 and 10.9 for seeds from capsules harvested at 151 and 94 days after pollination and cultured on basal medium + 2 g/L peptone in C. triplicata × C. masuca and its reciprocal, respectively.

I International Orchid Symposium

S.-J. Lee, F.-S. Liao, S.-H. Cheng

asymbiotic, orchids, pollination, reciprocal crosses

https://doi.org/10.17660/ActaHortic.2010.878.28