Articles
ESTABLISHMENT AND OPTIMIZATION OF AN EFFICIENT IN VITRO REGENERATION SYSTEM OF ONCIDIUM, WILSONARA, ODONTOCIDIUM AND VUYLSTEKEARA
Article number
878_57
Pages
445 – 452
Language
English
Abstract
Hybrids of Oncidium and Odontoglossum are orchids with a wide spectrum of flower colors and patterns and also with increasing economic importance.
The objective of this study was to develop an efficient protocol for in vitro regeneration via somatic embryogenesis.
Starting from in vitro shoot cultures of the cultivars Oncidium Sweet Sugar SP 12 and Sharry Baby var. aureum OK, as well as the hybrids Wilsonara Stirling Tiger, Odontocidium Hansueli Isler and Vuylstekeara Cambria Plush, we established a reliable protocol for regeneration of somatic embryos.
The most suitable type of explants were leaf tips, on which the formation of protocorm-like bodies was observed in frequencies of 60% after 12 weeks.
The most efficient regeneration was obtained on medium containing 1 mg/L thidiazuron and cultured in complete darkness.
The objective of this study was to develop an efficient protocol for in vitro regeneration via somatic embryogenesis.
Starting from in vitro shoot cultures of the cultivars Oncidium Sweet Sugar SP 12 and Sharry Baby var. aureum OK, as well as the hybrids Wilsonara Stirling Tiger, Odontocidium Hansueli Isler and Vuylstekeara Cambria Plush, we established a reliable protocol for regeneration of somatic embryos.
The most suitable type of explants were leaf tips, on which the formation of protocorm-like bodies was observed in frequencies of 60% after 12 weeks.
The most efficient regeneration was obtained on medium containing 1 mg/L thidiazuron and cultured in complete darkness.
Publication
Authors
B. Raffeiner, M. Serek, T. Winkelmann
Keywords
embryogenesis, liquid culture, micropropagation, orchids, somatic, thidiazuron
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