Articles
IN VITRO CONSERVATION OF DENDROBIUM DRACONIS UNDER MINIMAL GROWTH CONDITIONS
Article number
878_20
Pages
179 – 183
Language
English
Abstract
A protocol for in vitro storage of Dendrobium draconis Rchb. f. under minimal growth conditions was established for the conservation of the genetic diversity of orchids.
The response to different modified Murashige and Skoog (MS) medium was examined using D. draconis collected from northern Thailand.
Seeds were cultured on MS medium supplemented with 20 g/L sucrose.
Two-month-old protocorms were transferred to minimal growth medium.
In vitro culture could effectively be con¬served at 25±2°C for 12 months on 1/4 strength MS medium without an intervening subculture.
The microplants on this medium exhibited very slow growth and rarely developed another shoot.
The culture was also free of contamination.
The conserved microplants were subsequently recovered on MS medium and multiplied normally in MS medium with 1.0 mg/L benzyladenine.
The rooted shoots were successfully transferred to the nursery.
The response to different modified Murashige and Skoog (MS) medium was examined using D. draconis collected from northern Thailand.
Seeds were cultured on MS medium supplemented with 20 g/L sucrose.
Two-month-old protocorms were transferred to minimal growth medium.
In vitro culture could effectively be con¬served at 25±2°C for 12 months on 1/4 strength MS medium without an intervening subculture.
The microplants on this medium exhibited very slow growth and rarely developed another shoot.
The culture was also free of contamination.
The conserved microplants were subsequently recovered on MS medium and multiplied normally in MS medium with 1.0 mg/L benzyladenine.
The rooted shoots were successfully transferred to the nursery.
Publication
Authors
N. Rangsayatorn, C. Jaiphet
Keywords
micropropagation, orchids, germplasm
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