Articles
MOLECULAR DIVERSITY OF LOCAL SPANISH SWEET CHERRY CULTIVARS DETERMINED BY SSR AND S-LOCUS ANALYSIS
Article number
1020_1
Pages
33 – 38
Language
English
Abstract
Sweet cherry cultivar re-planting in fruit orchards is reducing local genetic diversity and traditional cultivars have fallen into disuse.
To conserve this genetic diversity, it is necessary to collect, maintain, and characterize this autochthonous germplasm.
Previous genetic and morphological analyses of local varieties from eastern Spain have revealed the existence of a genetic pool that is well differentiated from most other cultivated materials.
In this work local cherry varieties collected from northern Spain, the Mediterranean coast, and the Balearic Islands as well as from Spanish eastern regions have been analyzed and compared by PCR and capillary electrophoresis detection using microsatellite markers and the S-locus.
This has allowed the determination of genotype identities as well as the study of the existing genetic diversity.
Microsatellite analysis grouped the cultivars in various distinct clusters that mostly correlate with their geographical origin. S-allele diversity revealed higher frequency of certain S-alleles as well as the unique presence of some S-alleles in most isolated geographical areas.
The results obtained will be useful for the management and conservation of this local Spanish sweet cherry germplasm.
To conserve this genetic diversity, it is necessary to collect, maintain, and characterize this autochthonous germplasm.
Previous genetic and morphological analyses of local varieties from eastern Spain have revealed the existence of a genetic pool that is well differentiated from most other cultivated materials.
In this work local cherry varieties collected from northern Spain, the Mediterranean coast, and the Balearic Islands as well as from Spanish eastern regions have been analyzed and compared by PCR and capillary electrophoresis detection using microsatellite markers and the S-locus.
This has allowed the determination of genotype identities as well as the study of the existing genetic diversity.
Microsatellite analysis grouped the cultivars in various distinct clusters that mostly correlate with their geographical origin. S-allele diversity revealed higher frequency of certain S-alleles as well as the unique presence of some S-alleles in most isolated geographical areas.
The results obtained will be useful for the management and conservation of this local Spanish sweet cherry germplasm.
Publication
Authors
A.M. Cachi, R. Gella, A. Wünsch, M. López-Corrales, A. Arbeloa
Keywords
Prunus avium, genetic diversity, local germplasm, microsatellite, S-alleles
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