Key factors affecting in vitro conservation and rapid propagation of wild ornamental Gentiana

Wild Gentiana rhodantha, Gentiana arethusae, Gentiana rubicunda, and Gentiana duclouxii with high ornamental value were discovered and collected in low and middle-altitude regions and chosen as subjects to explore key technologies for in vitro conservation of wild Gentiana and to establish a rapid propagation system, in order to support its utilization and breeding new cultivars.
Suitable explants for the sterile material’s establishment, the best concentrations and combinations of hormones on adventitious bud induction and rooting, as well as the influence of pH on the growth of Gentiana were explored.
The results showed that the best explants for G. rhodantha and G. rubicunda is an apical bud and stem with an axillary bud, the best explant for G. arethusae is rootstock, and the best explant for G. duclouxii is immature seed.
The optimal medium for propagation is MS + 2.0 mg L^-1 6-BA + 0.10 mg L^-1 NAA for G. rhodantha, G. rubicunda and G. duclouxii, and MS + 2.0 mg L^-1 6-BA + 0.20 mg L^-1 for G. arethusae. The optimal medium for strengthening young plants is MS + 1.0 mg 
L^-1 6-BA + 0.10 mg L^-1 NAA, and the optimal medium for rooting is MS + 0.1 mg L^-1 NAA + 0.2 g L^-1 activated carbon.
A pH of 5.5 in the medium is good for Gentiana, and too high or low of a pH will disturb its growth.
In conclusion, the suitable explants differ for different Gentiana, and the use of suitable explants is essential for the establishment of sterile material.
Preventing water loss and utilizing a suitable media are the key factors for in vitro culturing and rapid propagation of the wild ornamental Gentiana.

II International Symposium on Germplasm of Ornamentals

H.Z. Wu, Y. Xiao, S.L. He, Q. Shao, S.Y. Zhang, J.Z. Wu

wild ornamental Gentiana, rapid propagation, in vitro conservation

https://doi.org/10.17660/ActaHortic.2017.1185.24