Articles
Deep sequencing-based transcriptome profiling analysis of Rhododendron latoucheae revealed insights into secondary metabolite biosynthesis
Article number
1185_26
Pages
197 – 208
Language
English
Abstract
Rhododendron latoucheae, a fragrant evergreen species of Rhododendron that originates from China, is a well-known landscape tree with important medicinal value.
Until now, there have been no molecular biology studies or genetic database resources of R. latoucheae, which makes it difficult to study the molecular biology of R. latoucheae. We used illumina RNA-seq technology for the leaves and flowers of R. latoucheae and have assembled an almost 7.77 GB database, containing 80,660 unigenes.
In the gene expression profiling analysis, the number of transcripts with GO annotation was 26,212. All unigenes are involved in 3 categories: biological process, cellular component, and molecular function.
There were 58 GO-terms, in which the dominant terms were ‘metabolic process’ (12,314, or 46.97%), ‘cellular process,’ (12,604, or 48.08%), and ‘binding’ (13,052, or 49.79%). Ten thousand two hundred and ninety unigenes were mapped to 273 biological pathways in the Kyoto Encyclopedia of Genes and Genomes pathway database, in which unigenes involved in lipid metabolism, biosynthesis of plant hormones, biosynthesis of phenylpropanoids, terpenoids and steroids, flavone and flavonol, and Isoflavonoid were annotated.
Based on the physiological characteristics of R. latoucheae, this paper preliminarily analyzed the unigenes involved in fragrance, pigments, and alkaloid in the secondary metabolites biosynthesis.
This data represents abundant messages about transcripts and provides valuable genome data sources in the molecular biology of R. latoucheae.
Until now, there have been no molecular biology studies or genetic database resources of R. latoucheae, which makes it difficult to study the molecular biology of R. latoucheae. We used illumina RNA-seq technology for the leaves and flowers of R. latoucheae and have assembled an almost 7.77 GB database, containing 80,660 unigenes.
In the gene expression profiling analysis, the number of transcripts with GO annotation was 26,212. All unigenes are involved in 3 categories: biological process, cellular component, and molecular function.
There were 58 GO-terms, in which the dominant terms were ‘metabolic process’ (12,314, or 46.97%), ‘cellular process,’ (12,604, or 48.08%), and ‘binding’ (13,052, or 49.79%). Ten thousand two hundred and ninety unigenes were mapped to 273 biological pathways in the Kyoto Encyclopedia of Genes and Genomes pathway database, in which unigenes involved in lipid metabolism, biosynthesis of plant hormones, biosynthesis of phenylpropanoids, terpenoids and steroids, flavone and flavonol, and Isoflavonoid were annotated.
Based on the physiological characteristics of R. latoucheae, this paper preliminarily analyzed the unigenes involved in fragrance, pigments, and alkaloid in the secondary metabolites biosynthesis.
This data represents abundant messages about transcripts and provides valuable genome data sources in the molecular biology of R. latoucheae.
Authors
W. Xing, M. Cai, Y. Chai, W. Zeng, X. Jin
Keywords
Rhododendron latoucheae, transcriptome, gene annotation, secondary metabolites biosynthesis
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