Articles
Plant genome editing made efficient and easy: targeted mutagenesis using the CRISPR/Cas system
Article number
1185_27
Pages
209 – 214
Language
English
Abstract
Genome editing is a standard plant breeding and transgenic method to reveal gene function and improve characteristics.
CRISPR/Cas9, a convenient, precise, and efficient genome editing technology, was recently developed and is a specific gene structure found in the genome of bacteria and archaea.
It is the immune system of bacteria and archaea involved in destroying phage and exogenous plasmids.
These sequence-specific nucleasea can recognize and cleave the specific sequence at target loci to generate double-strand breaks (DSBs), which are repaired by tow mechanisms: homologous recombination (HR) and non-homologous end joining (NHEJ). This system has been successfully used in rice, wheat, maize, sorghum, Arabidopsis, tobacco, sweet orange, and tomato using transient expression assays, including protoplast transformation and leaf agroinfiltration, according to recent studies.
However, there are no reports that suggest CRISPR/Cas9 is a useful tool for targeted mutagenesis of the genome of ornamental plants.
In this view, we summarize and discuss the advantages and further improvement aspects of this technology in recent applications of CRISPR/Cas9 in plants, in order to provide some references for the genome editing of ornamental plants.
CRISPR/Cas9, a convenient, precise, and efficient genome editing technology, was recently developed and is a specific gene structure found in the genome of bacteria and archaea.
It is the immune system of bacteria and archaea involved in destroying phage and exogenous plasmids.
These sequence-specific nucleasea can recognize and cleave the specific sequence at target loci to generate double-strand breaks (DSBs), which are repaired by tow mechanisms: homologous recombination (HR) and non-homologous end joining (NHEJ). This system has been successfully used in rice, wheat, maize, sorghum, Arabidopsis, tobacco, sweet orange, and tomato using transient expression assays, including protoplast transformation and leaf agroinfiltration, according to recent studies.
However, there are no reports that suggest CRISPR/Cas9 is a useful tool for targeted mutagenesis of the genome of ornamental plants.
In this view, we summarize and discuss the advantages and further improvement aspects of this technology in recent applications of CRISPR/Cas9 in plants, in order to provide some references for the genome editing of ornamental plants.
Authors
Y.H. Gao, Y.K. Gao, L. Yuan, Q.X. Zhang
Keywords
CRISPR/ Cas9, plant, genome editing, targeted mutagenesis, ornamental plants
Online Articles (39)