Articles
A COMPARISON OF VESICLES OBSERVED IN CELLS OF SYMPTOMLESS HEALTHY FRUIT TREE WITH THOSE FOUND IN VIRUS/INFECTED PLANTS.
Article number
130_10
Pages
71 – 72
Language
Abstract
Vesicles previously reported to be virus associated were observed in healthy symptomless controls during comparative cytopathological studies of two fruit tree virus diseases: Necrotic Rusty Mottle (NRM) of Prunus avium L. and Dead Spur(DS) of Malus domestica Borkh.
Healthy symptomless trees for the NRM study were located in orchards near Salem, Oregon, in greenhouses at the Interregional Deciduous Tree Fruit Repository, Prosses.
Washington and in a screenhouse at the Oregon State University Botany and Plant Pathology Research Facility.
The DS control trees were located in orchards in the Wenatchee, Washington area and at the Tree Fruit Research Station in Wenatchee.
Samples for transmission electron microscopy were taken from naturally infected and healthy symptomless tress.
Leaf midribs and fruit peduncles were collected from April through July by dicing tissues directly into 4% glutaraldehyde and subsequently processing via standard TEM techniques.
Vesicles were observed in thin sections of vascular tissues.
They ranged in diameter from 44 mm to 116 mm (average 63 mm), contained either a dense central core, a fibrillar inner matrix, or appeared clear.
The vesicles were most abundant in immature tracheary elements, one to three cells away from the fascicular cambial initials.
These vesicles are identical to those reported either as virus particles or virus-associated particles.
This is the first report of such vesicles in xylem cells and in healthy, symptomless, virus indexed tissues.
Healthy symptomless trees for the NRM study were located in orchards near Salem, Oregon, in greenhouses at the Interregional Deciduous Tree Fruit Repository, Prosses.
Washington and in a screenhouse at the Oregon State University Botany and Plant Pathology Research Facility.
The DS control trees were located in orchards in the Wenatchee, Washington area and at the Tree Fruit Research Station in Wenatchee.
Samples for transmission electron microscopy were taken from naturally infected and healthy symptomless tress.
Leaf midribs and fruit peduncles were collected from April through July by dicing tissues directly into 4% glutaraldehyde and subsequently processing via standard TEM techniques.
Vesicles were observed in thin sections of vascular tissues.
They ranged in diameter from 44 mm to 116 mm (average 63 mm), contained either a dense central core, a fibrillar inner matrix, or appeared clear.
The vesicles were most abundant in immature tracheary elements, one to three cells away from the fascicular cambial initials.
These vesicles are identical to those reported either as virus particles or virus-associated particles.
This is the first report of such vesicles in xylem cells and in healthy, symptomless, virus indexed tissues.
Authors
E.R. Florance, H.R. Cameron, C.L. Parish
Keywords
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