Home > XII International Symposium on Fruit Tree Virus Diseases > AN INDIRECT ELISA USING A SINGLE ANTISERUM AND A GENERAL PURPOSE CONJUGATE

Articles

AN INDIRECT ELISA USING A SINGLE ANTISERUM AND A GENERAL PURPOSE CONJUGATE

Article number

130_30

Pages

179 – 182

Language

Abstract

An indirect ELISA is described in which virus is trapped with F(ab’)₂ fragments of immunoglobulin, obtained by pepsin digestion, and detected with intact immunoglobulin and a protein A-peroxidase conjugate specific for the Fc portion of the intact antibody.
The assay is especially useful for detecting heterologous reactions and for applications that do not justify the effort or expense of preparing individual virus-specific conjugates.

Publication

XII International Symposium on Fruit Tree Virus Diseases

Authors

A.N. Adams, D.J. Barbara, M.F. Clark

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1983.130.30

Online Articles (57)

1. AN HISTORICAL PERSPECTIVE

A.F. Posnette

2. EUROPEAN FIGHTS AGAINST FRUIT TREE VIRUSES AS ORGANIZED BY EPPO AND EEC

H. Rønde Kristensen

3. IDEAL SCHEMES AND ASSOCIATED PROBLEMS IN THE PRODUCTION, MAINTENANCE, MULTIPLICATION, DISTRIBUTION AND CERTIFICATION OF FRUIT CROPS

C.A.R. Meijneke

4. A NEW VIRUS ISOLATED FROM PEACH IN HUNGARY

M. Németh | M. Kölber | C. Kerlan | J. Dunez

5. ASSOCIATION OF APPLE STEM GROOVING VIRUS WITH A DECLINE OF PACKHAM’S TRIUMPH PEAR ON SEEDLING ROOTSTOCK

Van Z. Siebert | D.J. Engelbrecht

6. EXPERIMENTS WITH SHIROFUGEN STUNT VIRUS, A LATENT VIRUS OF SOUR CHERRY

L. Kunze

7. DEAD SPUR OF RED DELICIOUS APPLE

C.L. Parish | E.A. Stahly | E.R. Florance | M.W. Williams

8. SWOLLEN SHOOT DISEASE OF CACAO IN TOGO: EARLY DETECTION OF VIRUS STRAINS IN THE TREES

M. Partiot | A.F. Posnette

9. X-DISEASE IN NECTARINE ASSESSMENT BY AERIAL PHOTOGRAPHY.

L.D. Lathrop | L.B. Forer | D. Bingaman | R.F. Stouffér

10. A COMPARISON OF VESICLES OBSERVED IN CELLS OF SYMPTOMLESS HEALTHY FRUIT TREE WITH THOSE FOUND IN VIRUS/INFECTED PLANTS.

E.R. Florance | H.R. Cameron | C.L. Parish

11. VIRUS ASSOCIATED WITH SEVERE DECLINE OF PLUM TREES IN POLAND

B. Zawadzka

12. A NUCLEIC ACID ASSOCIATED WITH NECROTIC RUSTY MOTTLE-DISEASED CHERRIES

D.L. Moore | H.R. Cameron

13. EFFECT OF PRUNUS VIRUSES ON GROWTH AND YIELD OF ITALIAN PRUNE TREES.

H.R. Cameron

14. INDEXING AND INCREASE METHOD OF “CLEAN” TRUE – TO – TYPE APPLE AND PEAR STOCK

Z. Gatin | A. Saric

15. DISTRIBUTION OF CHLOROTIC LEAF SPOT VIRUS-INFECTED BUDS ON VARIOUS LENGTHS OF APPLE BUDSTICKS IN SUCCESSIVE YEARS.

P.R. Fridlund

16. RESISTANCE OF SOME PRUNUS SPECIES TO THE PEACH LATENT MOSAIC VIRUS DISEASE (PLMV)

J.C. Desvignes

17. THE DEGREE OF SENSITIVITY OF SOME PLUM CULTIVARS AND HYBRIDS TO SHARKA (PLUM POX) VIRUS DISEASE

M. Rankovic

18. INHIBITING AND STIMULATING EFFECT OF PLANT SUBSTANCES ON VIRUS TRANSMISSION

B. György

19. CORRELATIONS AMONG SOME PROPERTIES OF DECIDUOUS FRUIT TREE VIRUSES

Paul R. Fridlund

20. BACK-TRANSMISSION OF APPLE STEM GROOVING VIRUS TO APPLE SEEDLINGS AND INDUCTION OF SYMPTOMS OF APPLE TOPWORKING DISEASE IN MITSUBA KAIDO (MALUS SIEBOLDII) AND KOBANO ZUMI (MALUS SIEBOLDII VAR. ARBORESCENS) ROOTSTOCKS

H. Yanase

21. VIRUS INFECTION OF WALNUTS IN FRANCE

R. Delbos | C. Kerlan | J. Dunez | M. Lansac | F. Dosba | E. Germain

22. HYPERSENSITIVITY OF WOODY INDICATORS FOR APPLE STEM GROOVING VIRUS

F.A. van der Meer

23. SUGAR CRAB, AN EFFECTIVE INDICATOR FOR DAPPLE APPLE.

R.F. Stouffer

24. COMPARATIVE STUDY OF SOME HERBACEOUS HOSTS FOR PLUM POX AND APPLE CHLOROTIC LEAF SPOT VIRUSES.

D. Sutic

25. ELISA DETECTION OF ILAR VIRUSES IN FRUIT TREES BY ANTISERA HAVING STRONG HETEROLOGOUS REACTIONS

R. Casper

26. USE OF ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR VIRUS DETECTION ON STONE FRUIT TREES IN SPAIN.

M. Cambra | G. Llácer | C. Pérez de Sanromán

27. THE DETECTION OF PLUM POX VIRUS (PPV) BY INDICATOR PLANTS AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

G. Hamdorf

28. ROUTINE TESTING OF ENGLISH WALNUT MOTHER TREES AND GROUP TESTING OF SEEDS BY ELISA FOR DETECTION OF CHERRY LEAF ROLL VIRUS INFECTION

M. Kölber | M. Németh | P. Szentiványi

29. IMMUNOELECTRONMICROSCOPY FOR VIRUS DETECTION AND VIRUS STRAIN IDENTIFICATION

C. Kerlan | B. Mille | J. Dunez

30. AN INDIRECT ELISA USING A SINGLE ANTISERUM AND A GENERAL PURPOSE CONJUGATE

A.N. Adams | D.J. Barbara | M.F. Clark

31. POTENTIAL OF RIBAVIRIN FOR TREE FRUIT VIRUS INHIBITION

A.J. Hansen | L. Green

32. SHOOT-TIP GRAFTING IN VITRO FOR ELIMINATION OF VIRUSES IN PEACH PLANTS (PRUNUS PERSICA BATSCH).

L. Navarro | G. Llácer | M. Cambra | J.M. Arregui | J. Juárez

33. VIROID-LIKE RNA ASSOCIATED WITH APPLE SCAR SKIN (OR DAPPLE APPLE) DISEASE

H. Koganezawa | H. Yanase | T. Sakuma

34. SURVEY FOR TOMATO RINGSPOT VIRUS IN APPLE ORCHARDS IN EASTERN NEW YORK

D.A. Rosenberger | D. Gonsalves | J.N. Cummins | M.B. Harrison

35. RESEARCH ON POME FRUIT ALTERATIONS REFERABLE TO VIRUS INFECTIONS

E. Refatti | R. Osler

36. GROWTH, YIELD AND FRUIT QUALITY OF VIRUS-INFECTED AND VIRUS-FREE GOLDEN DELICIOUS APPLE TREES, 1968 – 1982

H.J. van Oosten | C.A.R. Meijneke | H. Peerbooms

37. PRUNE DWARF VIRUS IN PRUNUS AVIUM L. AFFECTED BY FLAT LIMB AND STEM PITTING

F. Gualaccini | M. Barba

38. OLD BELGIAN CULTIVARS OF APPLE AND PEAR AS A SOURCE OF RESISTANCE TO DISEASE

C. Maroquin | C. Populer

39. TETRACYCLINE OR ZINC SULFATE TO CONTROL APPLE PROLIFERATION

J.A. Sánchez-Capuchino | R. Casanova | G. Llácer

40. EFFECTS OF TETRACYCLINE INJECTIONS AGAINST APPLE PROLIFERATION

C. Schmid

41. SOME FACTORS THAT LIMIT THE USEFULNESS OF ELISA RESULTS IN EFFORTS TO CONTROL SPREAD OF RUGOSE MOSAIC DISEASE IN SWEET CHERRY ORCHARDS.

G.I. Mink

42. THE OCCURRENCE AND SOME PROPERTIES OF THREE CARLAVIRUSES INFECTING HOP PLANTS (HUMULUS LUPULUS) IN ENGLAND

A.N. Adams | D.J. Barbara | M.F. Clark

43. OBSERVATIONS ON APRICOT CHLOROTIC LEAF ROLL (ACLR) : SENSITIVENESS OF DIFFERENT PRUNUS SPECIES, DETECTION, SPREAD IN PLUM ORCHARDS

J.C. Desvignes | D. Cornaggia

44. PROBLEMS ASSOCIATED WITH THE INTRODUCTION OF VIRUS- AND MYCOPLASMA-FREE APPLE TREES TO NEW ZEALAND ORCHARDS

G.A. Wood

45. THE AUSTRALIAN FRUIT VARIETY FOUNDATION AND ITS ROLE IN SUPPLYING VIRUS-TESTED PLANTING MATERIAL TO THE FRUIT INDUSTRY

P.R. Smith

46. TESTING FOR VIRUS IN REGISTERED SEED AND SCION ORCHARD BLOCKS IN WASHINGTON

Murit D. Aichele

47. INCIDENCE OF POLLEN-BORNE VIRUSES IN COMMERCIAL PRUNUS SEED LOTS.

G.I. Mink

48. THE NEED FOR AN INTERNATIONAL CERTIFICATION SCHEME OF IMPROVED TREE FRUIT PROPAGATION MATERIAL FOR DEVELOPING COUNTRIES

L. Chiarappa

49. SUSCEPTIBILITY OF STONE FRUIT TREES TO THE JAPANESE PLUM-TREE DECLINE CAUSAL AGENT.

L. Giunchedi | C. Poggi-Pollini | R. Credi

50. THE ASSOCIATION OF CHERRY LEAF ROLL VIRUS WITH BIRCH AND WALNUT POLLEN

P.R. Massalski | J.I. Cooper

51. ADDITIONAL EVIDENCE ON SEED TRANSMISSION OF PLUM POX VIRUS IN APRICOT, PEACH AND PLUM PROVED BY ELISA

M. Németh | M. Kölber

52. RASP LEAF AND DECLINE OF SWEET CHERRY IN AVELLINO PROVINCE

A. Ragozzino | F.P. D'Errico | C. De Vincentiis

53. EFFECT OF PRUNUS NECROTIC RINGSPOT VIRUS (PNRV) AND PRUNE DWARF VIRUS (PDV) ON SOME BIOLOGICAL PROPERTIES OF PEACH*

M.L. Topchiiska

54. ELECTRON MICROSCOPY DETECTION OF APPLE RUBBERY WOOD PATHOGENS AND COBALTO-THERAPY OF INFECTED FRUIT TREES

N. Minoiu | C. Craciun

55. THE EPIDEMIOLOGY OF PLUM POX VIRUS IN MOLDAVIA.^*

T.D. Verderevskaja | A.V. Andreev | B.V. Vereshjagin | A.I. Polinkovsky

56. MYCOPLASMA-LIKE BODIES ASSOCIATED WITH BLOSSOM ANOMALLY.

E.R. Florance | C.L. Parish

57. DETECTION OF VIRUS AND VIRUS LIKE DISEASES OF FRUIT TREES. INDEXING TECHNIQUES. INDICATOR RANGE

.