Articles
A NEW VIRUS ISOLATED FROM PEACH IN HUNGARY
Article number
130_4
Pages
33 – 46
Language
Abstract
In Hungary, from a peach tree showing leaf symptoms, a virus was isolated.
The virus was transmitted by grafting onto several woody plants /peach GF 305 and other Prunus species/ and mechanically onto different herbaceous hosts : Chenopodium amaranticolor is the best local lesion host plant ; C. quinoa and Nicotiana clevelandii are good propagation hosts.
Two suitable purification methods were developed : one used clarification by diethylether and carbon tetrachloride followed by two cycles of high and low speed centrifugation.
This method allowed obtention of partially purified virus suspensions used for preparation of a specific antiserum.
In the second method, after clarification by ammonium sulfate and PEG precipitation the virus was purified through two cycles of high speed centrifugation on sucrose gradients.
Particles sedimented as one single infectious component.
They are isometric with a diameter about 35 nm ; apparently they contained a single nucleic acid molecule.
No serological relationships were found with 17 viruses or strains using different serological techniques/gel diffusion, latex, ELISA, IEM/ applied either to crude sap or to purified virus suspensions.
Further investigations have to be carried out, but the results obtained allow to consider the virus isolated from peach trees as a new virus.
This virus was back inoculated from C. quinoa onto peach GF 305 seedlings and the typical symptoms developed which demonstrated the role of this virus in symptom expression.
Further investigations will complete the characterization of the virus.
The virus was transmitted by grafting onto several woody plants /peach GF 305 and other Prunus species/ and mechanically onto different herbaceous hosts : Chenopodium amaranticolor is the best local lesion host plant ; C. quinoa and Nicotiana clevelandii are good propagation hosts.
Two suitable purification methods were developed : one used clarification by diethylether and carbon tetrachloride followed by two cycles of high and low speed centrifugation.
This method allowed obtention of partially purified virus suspensions used for preparation of a specific antiserum.
In the second method, after clarification by ammonium sulfate and PEG precipitation the virus was purified through two cycles of high speed centrifugation on sucrose gradients.
Particles sedimented as one single infectious component.
They are isometric with a diameter about 35 nm ; apparently they contained a single nucleic acid molecule.
No serological relationships were found with 17 viruses or strains using different serological techniques/gel diffusion, latex, ELISA, IEM/ applied either to crude sap or to purified virus suspensions.
Further investigations have to be carried out, but the results obtained allow to consider the virus isolated from peach trees as a new virus.
This virus was back inoculated from C. quinoa onto peach GF 305 seedlings and the typical symptoms developed which demonstrated the role of this virus in symptom expression.
Further investigations will complete the characterization of the virus.
Authors
M. Németh, M. Kölber, C. Kerlan, J. Dunez
Keywords
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