Articles
COMPARATIVE STUDY OF SOME HERBACEOUS HOSTS FOR PLUM POX AND APPLE CHLOROTIC LEAF SPOT VIRUSES.
In mixed infection some plum trees showed less susceptibility to PPV or their fruits were tolerant to this virus.
To investigate this kind of possible interference it was necessary to find suitable herbaceous hosts, susceptible to both viruses.
In order to discover such hosts it was necessary to verify the susceptibility to different herbaceous plants to these viruses.
Four PPV isolates (N, Y, N/Y, SPr) and three CLSV isolates (Fr, YuPr, YuSc) were included in these experiments.
The identity of these isolates was primarly verified by means of biotest (indicator plants) and ELISA test.
In comparative inoculations by CLSV isolates the following herbaceous plants were infected: Caryophyllaceae: Agrostema gitago, Silene inflata, and Stellaria media; Compositae: Senecio sylvaticus, Labaiatae: Stachys recta; Ranunculaceae: Ranunculus arvensis, R.muricatus; Rubiaceae: Gallium aparine, and Scrophulariaceae: Digitalis lanata and Mimulus variegatus.
The successful transmission of virus isolates in these plants was confirmed by means of biotest but not yet with ELISA test which application in this experiment is in progress.
Three of PPV isolates (N, Y, N/Y) infected also some of these plants: A. gitago, S. media, S. sylvaticus, S. recta, R. arvensis, R. muricatus, R. sceleratus, D. lanata and M. variegatus.
One PPV isolate (SPr) until now infected only S. media and R. muricatus.
It seems that this isolate belongs to the mild PPV strains which was detected in infected plum trees with CLSV whose fruit are tolerant to PPV. The infection of these plants was successfully verified using biotest and in most casa ELISA test also.
The common hosts react to the PPV and CLSV differently but each of them could be of interest for investigation of these viruses or their strains in single as well as in mixed infections.