Articles
Development of plants resistant to Plum pox virus by interspecific hybridization between peach and other Prunus species
Article number
1352_16
Pages
121 – 126
Language
English
Abstract
Sharka, caused by Plum pox virus (PPV), is one of the most important disease of peach [P. persica (L.) Batsch] and other Prunus species.
PPV has been present in Turkey since 1968 however, contamination level is restricted in some regions depending on PPV strain.
PPV-D and PPV-T strains were identified mostly in residential gardens, whereas PPV-M was mostly detected in the commercial orchards.
The aim of this study is to identify genotypes that might be tolerant to PPV-M and to monitor the manner in which the resistance is transmitted to hybrid descendants by interspecific hybridization between peach and other Prunus species.
The apricot varieties Stark Early Orange’ and Harcot’, the almond cultivar Ferraduel’ and an accesion of P. davidiana identified as having less susceptibility to PPV were used in interspecific hybridizations to select hybrids to which this characteristic was transmitted.
Commercial peach cultivars like J.H.Hale’, Cresthaven’, Flored’, Persicum-Tan’, UFO-3′, Fantasia’, Persicum-ATEŞ’, Pitarina-CS46′, Crimson Baby’ and some rootstocks like Garnem, GF 677′ and Patrones-ARDA’ were used in hybridization studies.
The F1 individuals grown in insect-proof screen-house were submitted weekly to visual inspections and tested twice by DAS-ELISA and RT-PCR for the presence of PPV in one year period.
According to the all evaluation criteria (morphological, phenological and phomological), 17 individuals were selected for phenotyping studies.
They have been artificially inoculated by PPV-M strain for further scoring.
According to the pomological trait assessments and phenotyping analysis for the presence of PPV, most of the peach genotypes showed symptoms and were DAS-ELISA and RT-PCR positive, indicating their susceptibility to PPV. On the basis of phenotyping studies, 4 PPV tolerant peach hybrids from the hybrid families in which P. davidiana and Ferraduel’ were used as donors were selected for further breeding programs to observe their agronomic traits under the agro-ecological conditions of Adana-Turkiye.
PPV has been present in Turkey since 1968 however, contamination level is restricted in some regions depending on PPV strain.
PPV-D and PPV-T strains were identified mostly in residential gardens, whereas PPV-M was mostly detected in the commercial orchards.
The aim of this study is to identify genotypes that might be tolerant to PPV-M and to monitor the manner in which the resistance is transmitted to hybrid descendants by interspecific hybridization between peach and other Prunus species.
The apricot varieties Stark Early Orange’ and Harcot’, the almond cultivar Ferraduel’ and an accesion of P. davidiana identified as having less susceptibility to PPV were used in interspecific hybridizations to select hybrids to which this characteristic was transmitted.
Commercial peach cultivars like J.H.Hale’, Cresthaven’, Flored’, Persicum-Tan’, UFO-3′, Fantasia’, Persicum-ATEŞ’, Pitarina-CS46′, Crimson Baby’ and some rootstocks like Garnem, GF 677′ and Patrones-ARDA’ were used in hybridization studies.
The F1 individuals grown in insect-proof screen-house were submitted weekly to visual inspections and tested twice by DAS-ELISA and RT-PCR for the presence of PPV in one year period.
According to the all evaluation criteria (morphological, phenological and phomological), 17 individuals were selected for phenotyping studies.
They have been artificially inoculated by PPV-M strain for further scoring.
According to the pomological trait assessments and phenotyping analysis for the presence of PPV, most of the peach genotypes showed symptoms and were DAS-ELISA and RT-PCR positive, indicating their susceptibility to PPV. On the basis of phenotyping studies, 4 PPV tolerant peach hybrids from the hybrid families in which P. davidiana and Ferraduel’ were used as donors were selected for further breeding programs to observe their agronomic traits under the agro-ecological conditions of Adana-Turkiye.
Publication
Authors
E. Tanrıver, K. Çağlayan, M. Elçiçek, M. Yegül, P. Gök Güler, M. Gazel
Keywords
Plum pox virus-M (PPV-M) resistance, peach, P. davidiana, P. dulcis
Groups involved
Online Articles (87)