Articles
Effects of different LED wavelength absorption spectra on in vitro shoot proliferation, leaf anatomy, photosynthetic pigments and photosystem II photochemistry of GF677 rootstock
Article number
1352_62
Pages
453 – 462
Language
English
Abstract
Plant growth and development are strongly influenced by the spectrum of light in their environment.
In this study, the in vitro shoot proliferation, photosynthetic pigments, leaf anatomical traits and photosystem II photochemistry of GF677 rootstock (Prunus persica × Prunus amygdalus) were investigated under three light wavelength absorption spectra including 400-700 nm (mainly blue-green, white fluorescent light, WFL), 450-550 nm (mainly blue-red, LED1) and 450-660 nm (blue-green-yellow-orange-red, LED2) with 40 μmol m‑2 s‑1 intensity.
The culture medium used was the MS enriched with 3.1 μM BA, 0.05 μM NAA, 0.3 μM GA3, 20 g L‑1 sucrose (pH 5.8) and 6 g L‑1 agar (30 days, 16 h photoperiod, 22±2°C). WFL, LED1 and LED2 gave similar percentages of explants with new shoots formation (85.7-96.4%), shoot numbers or proliferation rates (1.9-2.2) and shoot heights (0.4-0.5 cm). LED1 presented the highest rate of stressed explants, with their leaf anatomy being severely affected, as leaves were thinner and with fewer layers of palisade and spongy parenchyma.
Under WFL, the highest significantly different values of explants fresh weight were observed while leaf anatomy remained unaffected.
Similarly, LED2 did not seem to negatively affect explants proliferation and their leaf anatomy.
Moreover, under WFL, the photoprotective energy dissipation (ΦNPQ) and the non-photochemical quenching (NPQ) were enhanced compared only to LED1. Photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids) did not differ among the applied treatments, with only a slight increase of carotenoids under WFL. The overall results showed that WFL is the most effective treatment, followed by LED2 as a more cost-beneficial technology that can contribute to an equally plant production of GF677.
In this study, the in vitro shoot proliferation, photosynthetic pigments, leaf anatomical traits and photosystem II photochemistry of GF677 rootstock (Prunus persica × Prunus amygdalus) were investigated under three light wavelength absorption spectra including 400-700 nm (mainly blue-green, white fluorescent light, WFL), 450-550 nm (mainly blue-red, LED1) and 450-660 nm (blue-green-yellow-orange-red, LED2) with 40 μmol m‑2 s‑1 intensity.
The culture medium used was the MS enriched with 3.1 μM BA, 0.05 μM NAA, 0.3 μM GA3, 20 g L‑1 sucrose (pH 5.8) and 6 g L‑1 agar (30 days, 16 h photoperiod, 22±2°C). WFL, LED1 and LED2 gave similar percentages of explants with new shoots formation (85.7-96.4%), shoot numbers or proliferation rates (1.9-2.2) and shoot heights (0.4-0.5 cm). LED1 presented the highest rate of stressed explants, with their leaf anatomy being severely affected, as leaves were thinner and with fewer layers of palisade and spongy parenchyma.
Under WFL, the highest significantly different values of explants fresh weight were observed while leaf anatomy remained unaffected.
Similarly, LED2 did not seem to negatively affect explants proliferation and their leaf anatomy.
Moreover, under WFL, the photoprotective energy dissipation (ΦNPQ) and the non-photochemical quenching (NPQ) were enhanced compared only to LED1. Photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids) did not differ among the applied treatments, with only a slight increase of carotenoids under WFL. The overall results showed that WFL is the most effective treatment, followed by LED2 as a more cost-beneficial technology that can contribute to an equally plant production of GF677.
Publication
Authors
V. Sarropoulou, I. Sperdouli, I.D. Adamakis, A. Konstantinidis, K. Grigoriadou
Keywords
micropropagation, Prunus persica × Prunus amygdalus, blue-green light
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