Articles
Distinct behaviors of the primary and secondary metabolome in peach and their connection to fruit quality development
Article number
1352_29
Pages
215 – 220
Language
English
Abstract
Preharvest factors, such as crop load (i.e., carbon supply) and canopy position (i.e., light availability) heavily influence peach fruit development, maturation and quality.
Few studies have investigated how these preharvest factors affect the metabolome, especially without the confounding variable of maturity.
Recent experiments were conducted to better understand the true impact of carbon supply and light availability on fruit quality development and the primary/secondary metabolism.
Fruit of equal maturity from two distinct carbon supply treatments (C-sufficient vs.
C-starved), and fruit from two distinct canopy positions (top vs. bottom), were assessed for their primary/secondary metabolite profiles through non-targeted gas/liquid chromatography mass-spectrometry.
In the crop load study, quality differences were minimal early in development, while primary metabolites were highly distinct between carbon supply (C-supply) treatments, priming the phenotypic differences present at harvest.
Minimal differences in the primary metabolome were detected at harvest between C-supply treatments, while several differences remained in the secondary metabolome.
Although, the majority and severity of the differences between C-supply treatments in the secondary metabolome were greatest early in development, like the primary metabolism, and characterized by up-accumulations of phenylpropanoids.
In distinct light environments, quality was superior in the top/exposed fruit, when compared to the bottom/shaded fruit, while minimal differences in the mesocarpRSQUOs primary metabolome were detected between treatments at harvest.
In contrast, primary and secondary metabolites were widely distinct in the exocarp of fruit originating from variable canopy positions.
Metabolic signatures of optimal growth conditions (e.g., sufficient C-supply, sun-exposure) and high fruit quality include: sorbitol, sucrose and flavonoids like, catechin, while signatures of inferior quality/conditions include: monosaccharides, amino acids and citric acid.
These preharvest factor studies demonstrate distinct responses of the primary and secondary metabolomes in peach mesocarp and exocarp, with connections to fruit quality development found in the shikimate pathway (primary metabolism) and phenylpropanoid pathway (secondary metabolism).
Few studies have investigated how these preharvest factors affect the metabolome, especially without the confounding variable of maturity.
Recent experiments were conducted to better understand the true impact of carbon supply and light availability on fruit quality development and the primary/secondary metabolism.
Fruit of equal maturity from two distinct carbon supply treatments (C-sufficient vs.
C-starved), and fruit from two distinct canopy positions (top vs. bottom), were assessed for their primary/secondary metabolite profiles through non-targeted gas/liquid chromatography mass-spectrometry.
In the crop load study, quality differences were minimal early in development, while primary metabolites were highly distinct between carbon supply (C-supply) treatments, priming the phenotypic differences present at harvest.
Minimal differences in the primary metabolome were detected at harvest between C-supply treatments, while several differences remained in the secondary metabolome.
Although, the majority and severity of the differences between C-supply treatments in the secondary metabolome were greatest early in development, like the primary metabolism, and characterized by up-accumulations of phenylpropanoids.
In distinct light environments, quality was superior in the top/exposed fruit, when compared to the bottom/shaded fruit, while minimal differences in the mesocarpRSQUOs primary metabolome were detected between treatments at harvest.
In contrast, primary and secondary metabolites were widely distinct in the exocarp of fruit originating from variable canopy positions.
Metabolic signatures of optimal growth conditions (e.g., sufficient C-supply, sun-exposure) and high fruit quality include: sorbitol, sucrose and flavonoids like, catechin, while signatures of inferior quality/conditions include: monosaccharides, amino acids and citric acid.
These preharvest factor studies demonstrate distinct responses of the primary and secondary metabolomes in peach mesocarp and exocarp, with connections to fruit quality development found in the shikimate pathway (primary metabolism) and phenylpropanoid pathway (secondary metabolism).
Publication
Authors
B.M. Anthony, D.G. Sterle, J.M. Chaparro, J.E. Prenni, I.S. Minas
Keywords
Prunus persica, dry matter content, index of absorbance difference, gas chromatography mass spectrometry, liquid chromatography mass spectrometry, non-targeted metabolomics, near-infrared spectroscopy
Groups involved
Online Articles (87)