Articles
Advance studies to develop biomarkers for water stresses tolerance in rootstocks
Article number
1352_22
Pages
163 – 168
Language
English
Abstract
Global warming is altering the precipitation regimens, which will lead to an increase in extreme events including drought in summer, and waterlogging in spring.
Thus, the modern cultivation systems require rootstocks environmentally adapted, with a controlled vigor for more sustainable orchards and more adapted to abiotic stress.
To confront these challenges in peach cultivation, two different studies were conducted: (i) a physiological, biochemical, and molecular characterization of the drought response in Garnem® almond × peach hybrid and Myrobalan P.2175′ plum submitted to different water deficit periods; and (ii) a transcriptomic analysis of the response to hypoxia of Felinem® almond × peach hybrid and Myrobalan P.2175′ plum to 24-h-root-hypoxia treatment.
The drought stress experiments evidenced that water-saver strategy of Myrobalan P.2175′ is not enough to cope with the drought stress.
This genotype triggers the activation of genes involved in signaling-cascade pathways, although without continuing toward a drought-tolerant response.
In contrast, Garnem®, with a water-spender strategy, initiates ABA-dependent pathways, which lead to the synthesis of osmoprotectants which allow an osmotic adjustment.
Furthermore, genes directly related to WUE, namely ERF023; ERECTA; and NF-YB3, as well as Myb25-like, a repressor of PP2C phosphatase, were selected as candidate biomarkers for use in Prunus rootstock breeding programs.
Likewise, we demonstrated that hypoxia tolerant Myrobalan P.2175′ plum represses secondary metabolism gene expression avoiding the waste of resources/energy as well as the upregulations of protein degradation genes, which led to structural adaptations conferring long-term tolerance to hypoxia.
In that work, three candidate genes involved in the oxygen sensing mechanism were identified as candidate biomarkers for hypoxia-tolerant selection, ERF74/RAP2.12, ACBP1/2 and HCR1.
Thus, the modern cultivation systems require rootstocks environmentally adapted, with a controlled vigor for more sustainable orchards and more adapted to abiotic stress.
To confront these challenges in peach cultivation, two different studies were conducted: (i) a physiological, biochemical, and molecular characterization of the drought response in Garnem® almond × peach hybrid and Myrobalan P.2175′ plum submitted to different water deficit periods; and (ii) a transcriptomic analysis of the response to hypoxia of Felinem® almond × peach hybrid and Myrobalan P.2175′ plum to 24-h-root-hypoxia treatment.
The drought stress experiments evidenced that water-saver strategy of Myrobalan P.2175′ is not enough to cope with the drought stress.
This genotype triggers the activation of genes involved in signaling-cascade pathways, although without continuing toward a drought-tolerant response.
In contrast, Garnem®, with a water-spender strategy, initiates ABA-dependent pathways, which lead to the synthesis of osmoprotectants which allow an osmotic adjustment.
Furthermore, genes directly related to WUE, namely ERF023; ERECTA; and NF-YB3, as well as Myb25-like, a repressor of PP2C phosphatase, were selected as candidate biomarkers for use in Prunus rootstock breeding programs.
Likewise, we demonstrated that hypoxia tolerant Myrobalan P.2175′ plum represses secondary metabolism gene expression avoiding the waste of resources/energy as well as the upregulations of protein degradation genes, which led to structural adaptations conferring long-term tolerance to hypoxia.
In that work, three candidate genes involved in the oxygen sensing mechanism were identified as candidate biomarkers for hypoxia-tolerant selection, ERF74/RAP2.12, ACBP1/2 and HCR1.
Publication
Authors
B. Bielsa, Á. Montesinos, M.J. Rubio-Cabetas
Keywords
almond × peach hybrid, candidate gene, drought, drought-adaption, hypoxia, root asphyxia
Groups involved
Online Articles (87)