Articles
Construction of a NIL collection of P. davidiana into the peach genetic background
Article number
1352_35
Pages
263 – 270
Language
English
Abstract
MAI is a breeding strategy used to increase the genetic variability of a species by introgressing new traits from closely related compatible species in a short period of time.
The proof of concept of the MAI strategy has been recently described using a cross between peach and almond.
This led to the identification of several almond genes with a potential use in peach breeding programs.
In this study, MAI was applied in interespecific crosses between peach and P. davidiana. Two BC1 populations (NT1Ba and NT1Ca), derived from a cross between the peach cultivar Nectatop and two P. persica x P. davidiana hybrids (Cadaman and Barrier), have been developed and are under analysis.
Both populations have been genotyped and individuals having a low number of introgressions of P. davidiana covering the whole P. davidiana genome were selected.
These individuals have been backcrossed to Nectatop and this BC2 progenies were or are being selected using a new LSQUO96.96 Genotyping IFC Fluidigm SNP array developed from the resequencing data of the parental lines.
In one population, 32 prILs (pre introgression lines; lines with 2-4 introgressed fragments) covering practically the entire genome of P. davidiana have been selected.
In this population, a new powdery mildew resistance gene was mapped.
For the other population, NT2Ca, the crosses have been done and next year a prILs collection will be selected.
These populations are being phenotyped for phenology, fruit quality, leaf morphology and resistance traits to identify useful genes from P. davidiana. QTL analysis identified several genomic regions involved in the genetic control of leaf shape, maturity date and bitter flesh taste, among others.
These materials will be useful for the identification of new alleles of interest for its use in peach breeding programs.
The proof of concept of the MAI strategy has been recently described using a cross between peach and almond.
This led to the identification of several almond genes with a potential use in peach breeding programs.
In this study, MAI was applied in interespecific crosses between peach and P. davidiana. Two BC1 populations (NT1Ba and NT1Ca), derived from a cross between the peach cultivar Nectatop and two P. persica x P. davidiana hybrids (Cadaman and Barrier), have been developed and are under analysis.
Both populations have been genotyped and individuals having a low number of introgressions of P. davidiana covering the whole P. davidiana genome were selected.
These individuals have been backcrossed to Nectatop and this BC2 progenies were or are being selected using a new LSQUO96.96 Genotyping IFC Fluidigm SNP array developed from the resequencing data of the parental lines.
In one population, 32 prILs (pre introgression lines; lines with 2-4 introgressed fragments) covering practically the entire genome of P. davidiana have been selected.
In this population, a new powdery mildew resistance gene was mapped.
For the other population, NT2Ca, the crosses have been done and next year a prILs collection will be selected.
These populations are being phenotyped for phenology, fruit quality, leaf morphology and resistance traits to identify useful genes from P. davidiana. QTL analysis identified several genomic regions involved in the genetic control of leaf shape, maturity date and bitter flesh taste, among others.
These materials will be useful for the identification of new alleles of interest for its use in peach breeding programs.
Publication
Authors
N. Zaracho, P. Arús, I. Eduardo
Keywords
interspecific cross, QTLs, prebreeding, prIL, powdery mildew
Groups involved
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