Articles
Short internodes-decline in ‘Neuburger’ – approximation by transcriptome analysis, qRT-PCR and methylation-sensitive longread-sequencing
Article number
1418_5
Pages
35 – 44
Language
English
Abstract
The grape cultivar ‘Neuburger’, native to Austria and still covering an area of 309 ha, has experienced a notable decline in cultivation due to signs of plant degeneration, such as shortened internodes leading to plant death.
Previous research suggested that gibberellin-enriched fertilizers could mitigate these symptoms, implicating the grapevine’s gibberellin metabolic pathways in the degeneration process.
This study employed a multifaceted approach involving gene expression analysis of gibberellin oxidases and degradation pathways, transcriptome analysis to outline complex metabolic pathways, qRT-PCR for further expression studies, and methylation-sensitive long-read sequencing to explore possible epigenetic factors involved in grapevine degeneration.
Plant material from ‘Neuburger’ cultivar, including normal and dwarf variant clones, were analyzed to identify genetic and epigenetic differences affecting plant development.
Transcriptomic and qRT-PCR analyses revealed significant differences in gene expression related to gibberellin metabolism, stress response pathways, and plant development.
Notably, the study identified significant regions of differential methylation associated with key proteins like the midchain alkane hydroxylase (MAH1) and chloroplast protein for growth and fertility (CGF1). MAH1 is crucial for the formation of the epicuticular wax layer, suggesting epigenetic influences on plant development.
Despite the high significance of the findings, the research opens up new avenues for further investigation into the genetic and epigenetic mechanisms underlying the complex degenerative symptoms in ‘Neuburger’ grapevines.
Previous research suggested that gibberellin-enriched fertilizers could mitigate these symptoms, implicating the grapevine’s gibberellin metabolic pathways in the degeneration process.
This study employed a multifaceted approach involving gene expression analysis of gibberellin oxidases and degradation pathways, transcriptome analysis to outline complex metabolic pathways, qRT-PCR for further expression studies, and methylation-sensitive long-read sequencing to explore possible epigenetic factors involved in grapevine degeneration.
Plant material from ‘Neuburger’ cultivar, including normal and dwarf variant clones, were analyzed to identify genetic and epigenetic differences affecting plant development.
Transcriptomic and qRT-PCR analyses revealed significant differences in gene expression related to gibberellin metabolism, stress response pathways, and plant development.
Notably, the study identified significant regions of differential methylation associated with key proteins like the midchain alkane hydroxylase (MAH1) and chloroplast protein for growth and fertility (CGF1). MAH1 is crucial for the formation of the epicuticular wax layer, suggesting epigenetic influences on plant development.
Despite the high significance of the findings, the research opens up new avenues for further investigation into the genetic and epigenetic mechanisms underlying the complex degenerative symptoms in ‘Neuburger’ grapevines.
Publication
Authors
R. Hack, F. Regner
Keywords
grapevine, gibberellins, qRT-PCR, RNA, sequencing, genetic marker, viticulture
Online Articles (52)