Articles
LED light quality affects biochemical composition of Lilium candidum adventitious bulbs in in vitro cultures on a cytokinin medium
Article number
1435_17
Pages
127 – 134
Language
English
Abstract
Lilium candidum L. (Madonna lily) is a bulbous geophyte that occurs naturally in the Mediterranean.
Its bulb and flower extracts have been used in folk medicine, and scientific research has confirmed the presence of compounds with health-promoting properties.
We evaluated biochemical composition of adventitious bulbs, contents of photosynthetic pigments (chlorophyll a + b and total carotenoids), soluble phenolics, and soluble sugars in bulblets grown under different light conditions.
In vitro cultures were carried out under eight different LED lights, with fluorescent lamp and darkness used as controls.
Murashige and Skoog medium was enriched with 5 µM 6-benzylaminopurine (BAP) and 0.5 µM 1-naphthaleneacetic acid (NAA). A mixture (7:3) of red and blue LED light (RB) most effectively stimulated accumulation of all tested pigments.
Lower but still high values were characteristic of bulblets from white LED light (WLED) and RB light enriched with a UV diode (RBUV). Synthesis of soluble phenolics was stimulated by blue LED light (B). In the case of soluble sugars, the light of a fluorescent lamp (FL) and WLED gave similarly high values.
Intermediate values were obtained under blue LED light.
LED light can therefore be used to modify the biochemical composition of L. candidum bulblets in in vitro cultures.
WLED light seems particularly suitable for this purpose.
Its bulb and flower extracts have been used in folk medicine, and scientific research has confirmed the presence of compounds with health-promoting properties.
We evaluated biochemical composition of adventitious bulbs, contents of photosynthetic pigments (chlorophyll a + b and total carotenoids), soluble phenolics, and soluble sugars in bulblets grown under different light conditions.
In vitro cultures were carried out under eight different LED lights, with fluorescent lamp and darkness used as controls.
Murashige and Skoog medium was enriched with 5 µM 6-benzylaminopurine (BAP) and 0.5 µM 1-naphthaleneacetic acid (NAA). A mixture (7:3) of red and blue LED light (RB) most effectively stimulated accumulation of all tested pigments.
Lower but still high values were characteristic of bulblets from white LED light (WLED) and RB light enriched with a UV diode (RBUV). Synthesis of soluble phenolics was stimulated by blue LED light (B). In the case of soluble sugars, the light of a fluorescent lamp (FL) and WLED gave similarly high values.
Intermediate values were obtained under blue LED light.
LED light can therefore be used to modify the biochemical composition of L. candidum bulblets in in vitro cultures.
WLED light seems particularly suitable for this purpose.
Authors
P. Pałka, B. Prokopiuk, B. Pawłowska
Keywords
photosynthetic pigments, soluble phenolics, soluble sugars
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