Articles
In vitro techniques for tulip micropropagation, virus eradication and tetraploid induction
Article number
1435_6
Pages
49 – 58
Language
English
Abstract
Improvement of biotechnological methods such as in vitro propagation can speed up breeding and provides new genotypes for the market.
Therefore, studies have been undertaken on the improvement of micropropagation method of tulip.
As a result of our research the cyclic multiplication of adventitious shoots of this geophyte was obtain by using 1-naphthaleneacetic acid (NAA) combined with cytokinins such as isopentenyladenine (iP) with addition of thidiazuron (TDZ) or meta-Topolin (mT). Moreover, the last micropropagation stage, formation of microbulbs was also significantly improved, that is very important because only bulbs are capable of rooting and further growth in soil.
It was found that modification (by replacement of TDZ with iP in a medium) and prolongation to 12 weeks of the last multiplication subculture, prior to cooling combined with the application of growth retardant enhanced shoot’s bulbing capacity.
Bulbing efficiency was markedly increased by treatment with MeJA at the last phase of this process, 6 weeks after the end of cooling shoots.
Due to the sever virus infection of several tulip cultivars, one of the aims of our research was to develop the in vitro method of virus eradication from plant material.
Virus elimination from the totally infected tulip genotypes was possible due to application of ribavirin for in vitro chemotherapy.
Currently in tulip, the polypoid cultivars are in high demand.
Therefore, during our study we developed in vitro method of tetraploid induction using herbicidal antimitotic agents such as oryzalin, amiprophos methyl (APM) and trifluralin as alternative to colchicine.
Therefore, studies have been undertaken on the improvement of micropropagation method of tulip.
As a result of our research the cyclic multiplication of adventitious shoots of this geophyte was obtain by using 1-naphthaleneacetic acid (NAA) combined with cytokinins such as isopentenyladenine (iP) with addition of thidiazuron (TDZ) or meta-Topolin (mT). Moreover, the last micropropagation stage, formation of microbulbs was also significantly improved, that is very important because only bulbs are capable of rooting and further growth in soil.
It was found that modification (by replacement of TDZ with iP in a medium) and prolongation to 12 weeks of the last multiplication subculture, prior to cooling combined with the application of growth retardant enhanced shoot’s bulbing capacity.
Bulbing efficiency was markedly increased by treatment with MeJA at the last phase of this process, 6 weeks after the end of cooling shoots.
Due to the sever virus infection of several tulip cultivars, one of the aims of our research was to develop the in vitro method of virus eradication from plant material.
Virus elimination from the totally infected tulip genotypes was possible due to application of ribavirin for in vitro chemotherapy.
Currently in tulip, the polypoid cultivars are in high demand.
Therefore, during our study we developed in vitro method of tetraploid induction using herbicidal antimitotic agents such as oryzalin, amiprophos methyl (APM) and trifluralin as alternative to colchicine.
Authors
M. Podwyszyńska, D. Sochacki
Keywords
bulb formation, chemotherapy, tissue culture, Tulipa, virus elimination, somaclonal variation
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