Articles
Distant hybridization of Hemerocallis with Eremurus and Lycoris
Article number
1435_19
Pages
143 – 150
Language
English
Abstract
Hemerocallis spp. is a perennial plant with high ornamental and economic value.
However, long-term hybridization between closely related species has made it difficult to obtain innovative Hemerocallis germplasm.
Distant hybridization can greatly enrich genetic diversity.
Consequently, a four-year hybridization study was carried out with Hemerocallis, Eremurus, and Lycoris. Artificial pollination was conducted to overcome the pre-zygotic reproductive barrier; embryo rescue was carried out to overcome the post-zygotic barrier.
The interfamily hybrid was identified, cytological and phenotypic observations were performed.
Analysis of cross-compatibility revealed that reproductive isolation strongly hinders distant hybridization.
For the hybridization between Hemerocallis and Eremurus, a total of 752 Hemerocallis flowers were pollinated, the fruit set rate is 0-5.26%, and only obtained 1 hybrid embryo.
And 378 Eremurus flowers were pollinated, no fruit was acquired.
For the hybridization between Hemerocallis and Lycoris, a total of 1597 Hemerocallis flowers were pollinated.
The fruit set rate was 0.41% and 1 hybrid embryo was obtained.
And 1446 Lycoris flowers were pollinated, the fruit set rate is 0-61.82%, 121 hybrid embryos were obtained.
The pre-zygotic isolation between Hemerocallis and Eremurus was more severe than Hemerocallis and Lycoris. After 7 months of embryo rescue, an interfamily hybrid seedling was obtained.
The hybrid was identified by inter-simple sequence repeat (ISSR). The growth rate of interfamily hybrid seedlings was at least five weeks slower than that of Lycoris interspecific hybrid seedlings.
The hybrid owned the same chromosome number as the paternal plants (2n=22) and showed maternal overall phenotypic characteristics.
Distant hybridization between Hemerocallis and Lycoris exhibits severe reproductive isolation, but the hybrid seedlings can still be obtained.
This research lays the foundation for the future development of Hemerocallis breeding and distant hybridization.
However, long-term hybridization between closely related species has made it difficult to obtain innovative Hemerocallis germplasm.
Distant hybridization can greatly enrich genetic diversity.
Consequently, a four-year hybridization study was carried out with Hemerocallis, Eremurus, and Lycoris. Artificial pollination was conducted to overcome the pre-zygotic reproductive barrier; embryo rescue was carried out to overcome the post-zygotic barrier.
The interfamily hybrid was identified, cytological and phenotypic observations were performed.
Analysis of cross-compatibility revealed that reproductive isolation strongly hinders distant hybridization.
For the hybridization between Hemerocallis and Eremurus, a total of 752 Hemerocallis flowers were pollinated, the fruit set rate is 0-5.26%, and only obtained 1 hybrid embryo.
And 378 Eremurus flowers were pollinated, no fruit was acquired.
For the hybridization between Hemerocallis and Lycoris, a total of 1597 Hemerocallis flowers were pollinated.
The fruit set rate was 0.41% and 1 hybrid embryo was obtained.
And 1446 Lycoris flowers were pollinated, the fruit set rate is 0-61.82%, 121 hybrid embryos were obtained.
The pre-zygotic isolation between Hemerocallis and Eremurus was more severe than Hemerocallis and Lycoris. After 7 months of embryo rescue, an interfamily hybrid seedling was obtained.
The hybrid was identified by inter-simple sequence repeat (ISSR). The growth rate of interfamily hybrid seedlings was at least five weeks slower than that of Lycoris interspecific hybrid seedlings.
The hybrid owned the same chromosome number as the paternal plants (2n=22) and showed maternal overall phenotypic characteristics.
Distant hybridization between Hemerocallis and Lycoris exhibits severe reproductive isolation, but the hybrid seedlings can still be obtained.
This research lays the foundation for the future development of Hemerocallis breeding and distant hybridization.
Authors
Y. Lv, Y.K. Gao, Y.X. Zhou, Y.X. Wang, Y.X. Cui, J. Zhou
Keywords
interfamily hybridization, intergeneric hybridization, daylily, Lycoris, foxtail lily, embryo rescue, reproductive isolation
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