Articles
EFFECT OF PRECURSOR AND POTASSIUM NITRATE ON INDIRUBIN PRODUCTION IN SUSPENSION CULTURE OF POLYGONUM TINCTORIUM
Article number
390_13
Pages
103 – 106
Language
Abstract
Effect of precursor and potassium nitrate on the growth of Polygonum tinctorium and indirubin formation was investigated using shake flasks and air-lift bioreactors.
Cell growth was impaired by precursor addition at the level higher than 2 mM indole.
Indirubin formation increased when 20 mM of indole was added at the mid-point of exponential phase of cells.
In an air-lift bioreactor using nitrate depleted media fed-batch culture increased indirubin production by 40%, compared with the control.
Cell growth was impaired by precursor addition at the level higher than 2 mM indole.
Indirubin formation increased when 20 mM of indole was added at the mid-point of exponential phase of cells.
In an air-lift bioreactor using nitrate depleted media fed-batch culture increased indirubin production by 40%, compared with the control.
Authors
T. Kim, G.W. Bae, I.S. Chung, H.J. Lee, Y.A. Chae
Keywords
indirubin, fed-batch culture, air-lift bioreactor, indole
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