Articles
MICROPROPAGATION OF REHMANNIA GLUTINOSA AS MEDICINAL PLANT BY SHOOT TIP AND ROOT SEGMENT CULTURE
Article number
390_15
Pages
113 – 120
Language
Abstract
One hundred percent contamination of root segment cultures were observed with the use of surface sterilant due to systematic internal contamination.
Four kinds of antibiotics namely, carbenicillin, gentamicin, kanamycin and vancomycin at levels of 5≈50 mg/L were all ineffective to prevent contamination.
Shoot proliferation from the culture of shoot apical meristem and node-bud was obtained from the medium supplemented with 1 to 5 mg/L BA, 0.3 mg/L IAA and 3.0% suerose with 0.6–1.2% Bacto agar instead of Gelrite which induced high frequency of vitrified shoots.
Addition of activated charcoal at concentrations of 0.1–0.3%, markedly increased shoot growth and promoted formation and growth of roots, and reduced the frequencies of vitrification but inhibited shoot mtutiplication.
Addition of growth inhibitors such as ancymidol, ABA, chloromequat and PBZ had no effect in preventing vitrification and reducing growth and proliferation of shoots.
Successful in vivo rooting from in vitro produced shoots was obtained by soaking shoot base in 100 mg/L IBA solution for 15 to 60 minutes or by treating shoot base with 0.1% IBA rooting powder and planting in rooting medium composed of 1:1 vermiculite and perlite.
Four kinds of antibiotics namely, carbenicillin, gentamicin, kanamycin and vancomycin at levels of 5≈50 mg/L were all ineffective to prevent contamination.
Shoot proliferation from the culture of shoot apical meristem and node-bud was obtained from the medium supplemented with 1 to 5 mg/L BA, 0.3 mg/L IAA and 3.0% suerose with 0.6–1.2% Bacto agar instead of Gelrite which induced high frequency of vitrified shoots.
Addition of activated charcoal at concentrations of 0.1–0.3%, markedly increased shoot growth and promoted formation and growth of roots, and reduced the frequencies of vitrification but inhibited shoot mtutiplication.
Addition of growth inhibitors such as ancymidol, ABA, chloromequat and PBZ had no effect in preventing vitrification and reducing growth and proliferation of shoots.
Successful in vivo rooting from in vitro produced shoots was obtained by soaking shoot base in 100 mg/L IBA solution for 15 to 60 minutes or by treating shoot base with 0.1% IBA rooting powder and planting in rooting medium composed of 1:1 vermiculite and perlite.
Authors
K.Y. Paek, K.J. Yu, S.I. Park, N.S. Sung, C.H. Park
Keywords
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