EFFECT OF CO₂ ENRICHMENT AND SUGAR-FREE MEDIUM ON SURVIVAL AND GROWTH OF TURFGRASS REGENERANTS GROWN IN VITRO

In two turfgrass species, Festuca arundinacea Schreb. (tall fescue) and Zoysia japonica Steud. (zoysiagrass), regeneration culture systems using two types of bioreactors were developed.
Regenerants of tall fescue and zoysiagrass were efficiently produced by using an aeration-agitation type bioreactor and a rotating drum type bioreactor, respectively.
The regenerants of each species were harvested from the bioreactors and cultivated in vitro during the preparation stage either on a ¼ strength N6 gellan gum (4 g l^-1) medium without sucrose or with 30 g l^-1 sucrose, and under CO₂ concentration of 0.4 or 50 mmol mol^-1, a photoperiod of 24 h per day and a photosynthetic photon flux density of 125 μmol m^-2 s^-1. The shoot and root lengths and shoot and root dry weights of tall fescue regenerants and the root dry weight of zoysiagrass regenerants were greater on the medium with sucrose than those on the medium without sucrose, regardless of the CO₂ concentration.
The survival percentage, shoot number and shoot length of zoysiagrass regenerants growing on the medium without sucrose under 50 mmol mol^-1 of CO₂ were the highest among all the treatments.

International Symposium on Plant Production in Closed Ecosystems

Y. Seko, T. Kozai

bioreactor culture, micropropagation, photoautotrophic growth, tall fescue, zoysiagrass

https://doi.org/10.17660/ActaHortic.1996.440.105