Articles
RELATIONSHIP BETWEEN SHAPE OF CARROT SOMATIC EMBRYOS AND THEIR GROWTH AND DEVELOPMENT
Article number
440_96
Pages
549 – 553
Language
Abstract
The relationship between embryo morphology and their growth and development was investigated using carrot as a model plant.
Photographs of individual torpedo-shaped embryos were taken under a microscope.
Then, the embryos were transplanted onto fibrous polyester substrate immersed in quarter strength MS medium with 7.5 g/L sucrose.
Growth of the individual embryos was observed in 7-d intervals for 28 days.
About 70% of the embryos grew without withering, but only half of these developed into leafy plants.
Among the leafy plants, less than one-third showed normal growth and development.
Most of the embryos with normal growth and development had deeply-separated and well developed cotyledons.
Many of the embryos which had curved root parts or lumps, such as secondary embryos, in the body developed into proliferated plant clusters or withered and died.
These results suggested that somatic embryo morphological information could be used for selecting suitable embryos for conversion.
Photographs of individual torpedo-shaped embryos were taken under a microscope.
Then, the embryos were transplanted onto fibrous polyester substrate immersed in quarter strength MS medium with 7.5 g/L sucrose.
Growth of the individual embryos was observed in 7-d intervals for 28 days.
About 70% of the embryos grew without withering, but only half of these developed into leafy plants.
Among the leafy plants, less than one-third showed normal growth and development.
Most of the embryos with normal growth and development had deeply-separated and well developed cotyledons.
Many of the embryos which had curved root parts or lumps, such as secondary embryos, in the body developed into proliferated plant clusters or withered and died.
These results suggested that somatic embryo morphological information could be used for selecting suitable embryos for conversion.
Authors
Y. Ibaraki, T. Shimazu, K. Kurata
Keywords
conversion, cotyledon, morphology, somatic embryogenesis, transplant production
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